Transcriptome- Based Analysis of EPO Dependent Cfu-e to Erythroblast Development

Abstract 3188 To provide molecular insight into erythroid developmental programs, including EPO- regulated aspects, we have employed transcriptome-based approaches to analyze the stage-wise development of purified murine bone marrow- derived CFUe, proerythroblasts and maturing erythroblasts. In vivo...

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Bibliographic Details
Published in:Blood 2012-11, Vol.120 (21), p.3188-3188
Main Authors: Verma, Rakesh, Narayanan, Aishwarya, Kuhrt, David, Li, Lei, Su, Su, Asch, Ruth, Jachimowicz, Edward, Wojchowski, Don M.
Format: Article
Language:English
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Summary:Abstract 3188 To provide molecular insight into erythroid developmental programs, including EPO- regulated aspects, we have employed transcriptome-based approaches to analyze the stage-wise development of purified murine bone marrow- derived CFUe, proerythroblasts and maturing erythroblasts. In vivo and ex vivo, these progenitors develop as KitposCD71highTer119neg; KitnegCD71highTer119neg; and KitnegCD71highTer119pos cohorts (designated as E1, E2, E3 stages, respectively). In the context of EPO- modulation, stage E1 cells exhibited ∼250 EPO- regulated target genes. In stage-E2 proerythroblasts, in contrast, 750 transcripts proved to be EPO- regulated while stage-E3 erythroblasts exhibited only select EPO- modulated genes (
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V120.21.3188.3188