Quantitative Recovery of 14C-labeled Carbon Monoxide (14CO) From Viable Heme-labeled Red Blood Cells in the Rat

Long-term recovery of 14C-labeled carbon monoxide (14CO) from labeled, transfused red blood cells (RBC) was studied in buffalo rats. Donor RBC (labeled with 14C-2-glycine) were transfused into host rats, and the 14CO formed from degradation of labeled hemoglobin heme was collected over the next 110+...

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Bibliographic Details
Published in:Blood 1972-08, Vol.40 (2), p.257-260
Main Author: Landaw, Stephen A.
Format: Article
Language:English
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Summary:Long-term recovery of 14C-labeled carbon monoxide (14CO) from labeled, transfused red blood cells (RBC) was studied in buffalo rats. Donor RBC (labeled with 14C-2-glycine) were transfused into host rats, and the 14CO formed from degradation of labeled hemoglobin heme was collected over the next 110+ days. The heme-equivalent 14CO recovery in 13 animals averaged 102.1 ± 2.1% (mean ± SE) of activity in hemoglobin heme of donor RBC. This confirms that heme of circulating RBC destroyed by random hemolysis and senescence is quantitatively converted to CO.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V40.2.257.257