Source of Hydrogen Peroxide and of Chemiluminescence Observed in Activated Human Platelet Preparations

Human platelet suspensions can be observed to produce small amounts of H2O2 (0.04 nmoles H2O2/min/2.5 × 105 cells/cu mm) and measurable chemiluminescence when exposed to target particles for phagocytosis, such as latex spherules. Both H2O2 production and chemiluminescence are characteristic of phago...

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Bibliographic Details
Published in:Blood 1977-10, Vol.50 (4), p.597-602
Main Authors: Krinsky, Norman I., Scoon, Kristine L., Hardin, Judy C., Levine, Peter H.
Format: Article
Language:English
Subjects:
Adenosine Diphosphate - pharmacology
Blood Platelets - metabolism
Humans
Hydrogen Peroxide - blood
Latex - pharmacology
Luminescent Measurements
Microspheres
Neutrophils - metabolism
Staphylococcus aureus - metabolism
Thrombin - pharmacology
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Summary:Human platelet suspensions can be observed to produce small amounts of H2O2 (0.04 nmoles H2O2/min/2.5 × 105 cells/cu mm) and measurable chemiluminescence when exposed to target particles for phagocytosis, such as latex spherules. Both H2O2 production and chemiluminescence are characteristic of phagocytosing polymorphonuclear leukocytes (PMN) and analysis of the purified platelets indicates contamination by PMN at the level of 0.2%. The amount of H2O2 produced and the chemiluminescence observed can be duplicated by adding latex spheres to a preparation of PMN at a concentration equivalent to the contaminant in the platelet preparations. We conclude that the H2O2 produced and chemiluminescence observed from activated platelets is due to the presence of small amounts of contaminating PMN. These studies emphasize the importance of controlling for PMN contamination in studies of platelet biochemistry and physiology.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V50.4.597.597