Detection of Primitive Macrophage Progenitor Cells in Mouse Bone Marrow

A previously undetected population of macrophage progenitor cells with high proliferative potential (HPP-CFC; an average of 5 × 104 cells/colony) in nutrient agar cultures has been demonstrated in post-fluorouracil (FU) and fluorouracil plus endotoxin (FUEt) treated and normal mouse bone marrow, usi...

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Bibliographic Details
Published in:Blood 1979-12, Vol.54 (6), p.1446-1450
Main Authors: Bradley, T.R., Hodgson, G.S.
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Cells
Cell Differentiation
Cells, Cultured
Colony-Stimulating Factors - pharmacology
Culture Media
Fluorouracil - pharmacology
Macrophages - cytology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Spleen
Time Factors
Tissue Extracts - pharmacology
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Summary:A previously undetected population of macrophage progenitor cells with high proliferative potential (HPP-CFC; an average of 5 × 104 cells/colony) in nutrient agar cultures has been demonstrated in post-fluorouracil (FU) and fluorouracil plus endotoxin (FUEt) treated and normal mouse bone marrow, using a combination of colony-stimulating factors, pregnant uterus extract (PMUE) plus human spleen-conditioned medium (HUSPCM). Neither PMUE nor HUSPCM alone stimulated colony formation by the HPP-CFC. The incidences of HPP-CFC were 1 in 2380 nucleated cells in normal marrow, 1 in 380 for 10-day post-FU, and 1 in 118 in 8-day post-FUEt marrow cells. HPP-CFC were only depleted to 57% of normal at 2 days after FU treatment, whereas the cells responsive to PMUE alone (low proliferative potential, LPP-CFC) were depleted to 1.2%, indicating a marked difference in cycling status of the respective types of progenitor cells.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V54.6.1446.1446