The Effect of Prostaglandins E1 and E2 on Macrophage Progenitor Cells With High Proliferative Potential in Mouse Bone Marrow In Vitro

• Published in: Blood 1984-06, Vol.63 (6), p.1348-1352
• Main Authors: Kriegler, A.B., Bradley, T.R., Hodgson, G.S.
• Format: Article
• Language: English
• Subjects: Alprostadil; Animals; Bone Marrow Cells; Cell Division - drug effects; Dinoprostone; Fluorouracil - pharmacology; Humans; Macrophages - drug effects; Male; Mice; Mice, Inbred BALB C; Prostaglandins E - pharmacology; Stem Cells - drug effects
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V63.6.1348.1348

High proliferative potential macrophage progenitor cells (HPP-CFC) in 5-fluorouracil (FU) treated and normal mouse bone marrow (BM) have been shown to be less sensitive to inhibition of proliferation by prostaglandins of the E series (PGE) than low proliferative potential macrophage progenitor cells (LPP-CFC) in normal BM in agar cultures. The growth of large colonies (diameter >0.5 mm) derived from HPP-CFC in FU BM, which require a combination of macrophage colony-stimulating factor (CSF-1) plus a new growth factor called synergistic activity (SA), are inhibited by 50% in the presence of 5.5 x 10-6M PGE1 On the other hand, LPP-CFC in normal BM, which form smaller colonies (diameter ≤0.5 mm) in the presence of CSF-1 alone, require only 5 x 10-8M PGE1 for the same level of inhibition. Addition of appropriate concentrations of PGE1 to the agar culture assay should improve detection of HPP-CFC by inhibiting the proliferation of LPP-CFC. These observations suggest that the apparent negative feedback control of macrophage production by PGE operates largely on the LPP-CFC, which respond to CSF-1 alone, and is probably not involved in the regulation of the more primitive HPP-CFC.