Phorbol ester induces abnormal chronic lymphocytic leukemia cells to express features of hairy cell leukemia

We have investigated the relationship between chronic lymphocytic leukemia (CLL), hairy cell leukemia (HCL), and different normal B cell subsets: Mrbc+, T1+ and slgM+ tonsil cells; germinal center; mantle zone; and peripheral blood B lymphocytes. Both malignant and normal cells were incubated in vit...

Full description

Saved in:
Bibliographic Details
Published in:Blood 1985-11, Vol.66 (5), p.1035-1042
Main Authors: Caligaris-Cappio, F, Pizzolo, G, Chilosi, M, Bergui, L, Semenzato, G, Tesio, L, Morittu, L, Malavasi, F, Gobbi, M, Schwarting, R
Format: Article
Language:English
Subjects:
Acid Phosphatase - metabolism
Aged
B-Lymphocytes - classification
Biological and medical sciences
Bone Marrow Cells
Cells, Cultured
Embryonal Carcinoma Stem Cells
Hematologic and hematopoietic diseases
Humans
Leukemia, Hairy Cell - blood
Leukemia, Lymphoid - pathology
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Lymphocyte Activation
Male
Medical sciences
Middle Aged
Mitogens - pharmacology
Neoplastic Stem Cells - drug effects
Phorbol Esters - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have investigated the relationship between chronic lymphocytic leukemia (CLL), hairy cell leukemia (HCL), and different normal B cell subsets: Mrbc+, T1+ and slgM+ tonsil cells; germinal center; mantle zone; and peripheral blood B lymphocytes. Both malignant and normal cells were incubated in vitro with the phorbol ester 12-O-tetradecanoyl- phorbol-13-acetate (TPA) for 72 hours and the morphology, cytochemical profile, and surface markers were evaluated. The results show that CLL cells TPA-induced become indistinguishable from HCL by four independent criteria: the morphology; the cytoplasmic tartrate resistant acid phosphatase (TRAP) enzyme activity; the membrane positivity with anti- Leu M5 (SHCL3); and anti-Tac monoclonal antibodies which, in the uninduced state, react only with HCL. The features of TRAP and Tac positivity are also expressed (though in variable degree) by different normal B cell populations activated with TPA or pokeweed mitogen (PWM). It is concluded that HCL might represent an aberrantly activated variant of CLL (or of a CLL-related disorder).
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V66.5.1035.1035