Identification and Characterization of a Differentiation-Specific Antigen on Normal and Malignant Murine Erythroid Cells

Rauscher murine erythroleukemia cells grow continuously in vitro and undergo terminal differentiation in response to the physiological inducer erythropoietin. In the course of this developmental process they express many erythroid-specific markers. In order to investigate the expression of cell surf...

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Bibliographic Details
Published in:Blood 1987-01, Vol.69 (1), p.103-108
Main Authors: Bacon, E.Rena, Svtkowski, Arthur J.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
Antigens, Surface - analysis
Biological and medical sciences
Bone Marrow - immunology
Bone Marrow Cells
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Erythrocytes - cytology
Erythrocytes - immunology
Erythropoiesis - drug effects
Erythropoietin - pharmacology
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Hematopoietic Stem Cells - immunology
Leukemia, Erythroblastic, Acute - immunology
Leukemia, Erythroblastic, Acute - pathology
Mice
Molecular and cellular biology
Phenylhydrazines - pharmacology
Spleen - immunology
Time Factors
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Summary:Rauscher murine erythroleukemia cells grow continuously in vitro and undergo terminal differentiation in response to the physiological inducer erythropoietin. In the course of this developmental process they express many erythroid-specific markers. In order to investigate the expression of cell surface determinants during Rauscher cell differentia tion we generated monoclonal antibodies to uninduced cells. Using an anti-Rauscher cell monoclonal antibody, we have identified a cell surface determinant, designated ERY-1, that is present on normal murine erythroid cells. This determinant is apparently absent from the early progenitor BFU-E, but is present on the more mature progenitors CFU-E and CFC-E. It disappears during ery throid maturation and is absent from the mature erythro cyte. This pattern of ERY-1 expression is exhibited with remarkable fidelity during the erythropoietin-induced dif ferentiation of Rauscher cells. Such differentiation-specific expression of the ERY-1 determinant suggests that it may play a functional role in erythropoiesis.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V69.1.103.103