Human Granulocyte Colony-Stimulating Factor Specifically Binds to Murine Myeloblastic NFS-60 Cells and Activates Their Guanosine Triphosphate Binding Proteins/Adenylate Cyclase System

• Published in: Blood 1989-11, Vol.74 (7), p.2343-2348
• Main Authors: Matsuda, Satoru, Shirafuji, Naoki, Asano, Shigetaka
• Format: Article
• Language: English
• Subjects: Adenylyl Cyclases - metabolism; Animals; Biological and medical sciences; Cell Division; Cell Line; Cell Membrane - metabolism; Cell physiology; Cholera Toxin - pharmacology; Colony-Stimulating Factors - metabolism; Colony-Stimulating Factors - pharmacology; Enzyme Activation; Fundamental and applied biological sciences. Psychology; Granulocyte Colony-Stimulating Factor; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Substances - pharmacology; GTP-Binding Proteins - metabolism; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Triphosphate - metabolism; Macrophage Colony-Stimulating Factor; Mice; Molecular and cellular biology; Receptors, Cell Surface - metabolism; Receptors, Granulocyte Colony-Stimulating Factor; Responses to growth factors, tumor promotors, other factors; Signal Transduction; Thionucleotides - metabolism
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V74.7.2343.2343

The specific binding of human granulocyte colony-stimulating factor (G-CSF) to its receptors on NFS-60 cells acts as a primer for cellular proliferation. There are approximately 400 binding sites per cell, with a binding constant of about 100 pmol/L. Before the proliferative response, the affinity constant of the membrane particulate fraction to 35S-labeled guanosine triphosphate-gamma-S (35S-GTP7S) and the intracellular cyclic adenylate monophosphate (cAMP) level increased in the presence of G-CSF to about 2.5-fold and about fivefold higher, respectively, than the levels seen in the absence of G-CSF. The increases were time-dependent, with a peak occuring 15 minutes after the addition of G-CSF at 37°C. These findings suggest that, following the binding of the G-CSF to its surface receptors, the activation of the guanosine triphosphate (GTP)-binding protein/adenylate cyclase system may be involved in the proliferation of immature myeloid cells.