Synergistic effects of interleukin-1β and interleukin-3 on the expansion of human hematopoietic progenitor cells in liquid cultures

In the present study, we show that recombinant human interleukin-1 beta (rhIL-1 beta), which has no effect on the proliferation of human progenitor cells, has synergistic effects on the expansion of human progenitor cells induced by rhIL-3 in liquid cultures. The synergistic effects of rhIL-1 beta w...

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Bibliographic Details
Published in:Blood 1991-10, Vol.78 (8), p.1947-1953
Main Authors: KOBAYASHI, M, IMAMURA, M, GOTOHDA, Y, MAEDA, S, IWASAKI, H, SAKURADA, K, KASAI, M, MIYAZAKI, T
Format: Article
Language:English
Subjects:
Antibodies - physiology
Antigens, CD - analysis
Antigens, CD34
Biological and medical sciences
Cell Division - drug effects
Drug Synergism
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - immunology
Humans
Immunohematology
Indicator Dilution Techniques
Interleukin-1 - pharmacology
Interleukin-3 - pharmacology
Recombinant Proteins - pharmacology
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Summary:In the present study, we show that recombinant human interleukin-1 beta (rhIL-1 beta), which has no effect on the proliferation of human progenitor cells, has synergistic effects on the expansion of human progenitor cells induced by rhIL-3 in liquid cultures. The synergistic effects of rhIL-1 beta with rhIL-3 were observed in liquid cultures using not only fresh bone marrow mononuclear cells, but also selected populations of nonadherent cells, non-T nonadherent cells, and CD34-positive cells. Anti-granulocyte-macrophage colony-stimulating factor (anti-GM-CSF) antibody partially blocked the synergistic effects of rhIL-1 beta on the proliferation of colony-forming unit (CFU)-GM burst-forming unit-erythroid (BFU-E), and CFU-Mix in liquid cultures in the presence of rhIL-1 beta plus rhIL-3, suggesting that the synergistic effects of rhIL-1 beta plus rhIL-3 are explained in part by the secondary production of GM-CSF. Limiting dilution assays and liquid culture assays using CD34-positive cells indicate that rhIL-1 beta directly increases the numbers of colony-forming cells in liquid cultures. These results suggest that rhIL-1 beta has unique direct and indirect effects on the expansion of hematopoietic progenitor cells in liquid cultures.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V78.8.1947.1947