Blood Flow to Bone Marrow During Development of Anemia or Polycythemia in the Rat

We applied the radioactive microsphere method to follow the magnitude and time course (0 to 96 hours) of blood flow changes during development and recovery from anemia in awake rats. Blood flow was also monitored during a 96-hour period after polycythemia was induced (erythropoietin administered sub...

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Bibliographic Details
Published in:Blood 1992-02, Vol.79 (3), p.594-601
Main Authors: Iversen, Per Ole, Nicolaysen, Gunnar, Benestad, Haakon B.
Format: Article
Language:English
Subjects:
Anemia - physiopathology
Anemias. Hemoglobinopathies
Animals
Biological and medical sciences
Bone Marrow - blood supply
Bone Marrow - metabolism
Deoxyglucose - metabolism
Diseases of red blood cells
Erythropoiesis
Erythropoietin - administration & dosage
Hematologic and hematopoietic diseases
Iron Radioisotopes - metabolism
Male
Medical sciences
Oxygen Consumption
Polycythemia - physiopathology
Rats
Rats, Inbred Strains
Recombinant Proteins
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Summary:We applied the radioactive microsphere method to follow the magnitude and time course (0 to 96 hours) of blood flow changes during development and recovery from anemia in awake rats. Blood flow was also monitored during a 96-hour period after polycythemia was induced (erythropoietin administered subcutaneously [SC]). The possible influence of innervation was also examined. After a blood loss of approximately 50% (hypovolemia), blood flow to the femoral marrow tripled within 12 hours and remained elevated for the entire 96-hour period. The relative increase in blood flow to the femoral bone was even greater. Similar findings were obtained in rats with phenylhydrazine (PHZ) hemolytic anemia (normovolemia). Denervation had no detectable effect on the increased blood flow to either marrow or bone. The aug- mented blood flow during hemolytic anemia was accompanied by a doubling of the oxygen consumption rate by the marrow, while the glucose uptake was not detectably altered. Erythropoietin supplements (3 x 1,000 IU/kg, SC, 6-hour intervals) increased blood flow to the marrow by approximately 25% after 48 hours, and at 72 hours the blood flow had reached a value twice that obtained under control conditions. These results indicate that blood flow to bone marrow is highly variable and hormonally and/or locally regulated. This may have practical consequences for marrow transplantation technology and for administration of drug therapy to patients with insufficient bone marrow hematopoiesis.© 1992 by The American Society of Hematology. 0006-4971/92/7903-0018$3.00/0
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V79.3.594.594