In vivo administration of stem cell factor to mice increases the absolute number of pluripotent hematopoietic stem cells

We have examined the effects of administration of stem cell-factor (SCF) on the number and distribution of pluripotent hematopoietic stem cells (PHSC) in normal mice. Using the competitive repopulation assay we found that in vivo administration of SCF increases the absolute number of PHSC per mouse...

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Bibliographic Details
Published in:Blood 1993-07, Vol.82 (2), p.445-455
Main Authors: Bodine, David M., Seidel, Nancy E., Zsebo, Krisztina M., Orlic, Donald
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bone Marrow - chemistry
Bone Marrow Cells
Cell Count
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
DNA - analysis
Drug Resistance - genetics
Erythroid Precursor Cells - cytology
Fluorouracil - pharmacology
Fundamental and applied biological sciences. Psychology
Granulocytes - cytology
Hematopoietic Cell Growth Factors - pharmacology
Hematopoietic Stem Cells - cytology
Lymphocytes - cytology
Mice
Mice, Inbred C57BL
Molecular and cellular biology
Phenotype
Polyethylene Glycols
Proto-Oncogene Proteins - analysis
Proto-Oncogene Proteins c-kit
Recombinant Proteins - pharmacology
Spleen - cytology
Stem Cell Factor
Thymus Gland - chemistry
Transfection
Whole-Body Irradiation
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Summary:We have examined the effects of administration of stem cell-factor (SCF) on the number and distribution of pluripotent hematopoietic stem cells (PHSC) in normal mice. Using the competitive repopulation assay we found that in vivo administration of SCF increases the absolute number of PHSC per mouse threefold. The increased numbers of PHSC are found in the peripheral blood and spleen of the SCF-treated animals. The spleen and peripheral blood stem cells completely repopulated the erythroid, myeloid, and lymphoid lineages of irradiated or W/Wv hosts, similar to bone marrow PHSC. PHSC from the peripheral blood of SCF- treated mice have a lineage marker-negative, c-kit-positive phenotype that is indistinguishable from that of bone marrow PHSC. The increase in the absolute number of spleen PHSC is associated with efficient gene transfer to these cells without prior treatment with 5-fluorouracil. This is a US government work. There are no restrictions on its use..
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V82.2.445.445