A Bone Marrow Stromal-Derived Growth Factor, Interleukin-11, Stimulates Recovery of Small Intestinal Mucosal Cells After Cytoablative Therapy

• Published in: Blood 1994-01, Vol.83 (1), p.33-37
• Main Authors: Du, X.X., Doerschuk, C.M., Orazi, A., Williams, D.A.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Bone Marrow - drug effects; Bone Marrow Cells; Cell Division - drug effects; Fluorouracil - toxicity; Immunomodulators; Interleukin-11 - pharmacology; Intestinal Mucosa - drug effects; Intestinal Mucosa - radiation effects; Medical sciences; Mice; Mice, Inbred C3H; Pharmacology. Drug treatments; Stromal Cells - chemistry
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V83.1.33.33

The proliferation of epithelial cells lining the small intestinal mucosa may be regulated by microenvironmental signals leading to differentiation of precursor cells in the small intestinal crypts. Proliferation of hematopoietic cells within the hematopoietic microenvironment is known to be regulated by a growing number of glycoprotein growth factors in a hierarchial fashion. We studied the effects of administration of the microenvironment-derived hematopoietic growth factor interieukin-11 (IL-11) on mice given combination radiation/chemotherapy. Treatment of such mice with IL-11 led to significantly increased survival and evidence of rapid recovery of the small intestinal mucosa, which is severely damaged by these cytoxic agents. This recovery was associated with an increase in the mitotic index of crypt cells and an increased frequency of staining of these cells with a monoclonal antibody to proliferating cell nuclear antigen, a member of the cyclin family of nuclear antigens.