Role of Granulocyte-Macrophage Colony-Stimulating Factor and Granulocyte Colony-Stimulating Factor in the Development of an Acute Neutrophil Inflammatory Response in Mice

• Published in: Blood 1996-11, Vol.88 (10), p.3755-3764
• Main Authors: Metcalf, Donald, Robb, Lorraine, Dunn, Ashley R., Mifsud, Sandra, Rago, Ladina Di
• Format: Article
• Language: English
• Subjects: Animals; Ascitic Fluid - chemistry; Ascitic Fluid - cytology; Bacterial Infections - physiopathology; Biological and medical sciences; Bone Marrow - pathology; Caseins - toxicity; Chemotaxis, Leukocyte - physiology; Eosinophils; Fundamental and applied biological sciences. Psychology; Granulocyte Colony-Stimulating Factor - analysis; Granulocyte Colony-Stimulating Factor - physiology; Granulocyte-Macrophage Colony-Stimulating Factor - analysis; Granulocyte-Macrophage Colony-Stimulating Factor - physiology; Inflammation; Leukocyte Count; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred DBA; Mice, Knockout; Mice, Transgenic; Molecular and cellular biology; Neutrophils - physiology; Peritonitis - chemically induced; Peritonitis - physiopathology; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - genetics; Thioglycolates - toxicity
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V88.10.3755.bloodjournal88103755

The intraperitoneal injection into mice of casein preparations containing bacteria induced a rapid accumulation of neutrophils within 3 hours due to selective release of mature cells from the bone marrow. Significant increases in the concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) occurred in the peritoneal cavity during the process, but the intraperitoneal injection of neither CSF induced a significant accumulation of neutrophils and the coinjection of G-CSF and casein failed to enhance the neutrophil response. The lack of involvement of either CSF in the neutrophil migration was confirmed by the development of typical neutrophil exudates when casein was injected into mice with inactivation of the genes encoding GM-CSF, G-CSF, or the β-common chain of the GM-CSF receptor. However, preinjection of G-CSF increased the number of marrow neutrophils available for migration and did result in increased numbers of neutrophils in the peritoneal cavity after casein injection. Typical eosinophil inflammatory responses to the injection of casein or thioglycollate occurred in GM-CSF -/- mice but not in βc -/- mice, suggesting that interleukin-5 was necessary for this response.