Hydrogen peroxide is involved in collagen-induced platelet activation

In this study, we investigated whether (1) collagen-induced platelet aggregation is associated with a burst of H2O2, (2) this oxidant species is involved in the activation of platelets, and (3) the pathways of platelet activation are stimulated by H2O2. Collagen-induced platelet aggregation was asso...

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Bibliographic Details
Published in:Blood 1998-01, Vol.91 (2), p.484-490
Main Authors: PIGNATELLI, P, PULCINELLI, F. M, LENTI, L, GAZZANIGA, P. P, VIOLI, F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood coagulation. Blood cells
Blood Platelets - physiology
Collagen - pharmacology
Fundamental and applied biological sciences. Psychology
Humans
Hydrogen Peroxide - metabolism
Molecular and cellular biology
Platelet
Platelet Activation - drug effects
Signal Transduction - drug effects
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Summary:In this study, we investigated whether (1) collagen-induced platelet aggregation is associated with a burst of H2O2, (2) this oxidant species is involved in the activation of platelets, and (3) the pathways of platelet activation are stimulated by H2O2. Collagen-induced platelet aggregation was associated with production of H2O2, which was abolished by catalase, an enzyme that destroys H2O2. H2O2 production was not observed when ADP or thrombin were used as agonists. Catalase inhibited dose-dependently thromboxane A2 production, release of arachidonic acid from platelet membrane, and Inositol 1,4,5P3 (IP3) formation. In aspirin-treated platelets stimulated with high concentrations of collagen, catalase inhibited platelet aggregation, calcium mobilization, and IP3 production. This study suggests that collagen-induced platelet aggregation is associated with a burst of H2O2 that acts as a second messenger by stimulating the arachidonic acid metabolism and phospholipase C pathway.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.v91.2.484