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Cellular Localization of Orexin Receptors in Human Pituitary
Orexins-A and -B are hypothalamic peptides derived from a precursor called prepro-orexin and relationated with the stimulation of food intake. They act on G protein receptors named orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R), respectively. In the present study, we used RT-PCR and immunohis...
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| Published in: | The journal of clinical endocrinology and metabolism 2001-07, Vol.86 (7), p.3444-3447 |
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| container_end_page | 3447 |
| container_issue | 7 |
| container_start_page | 3444 |
| container_title | The journal of clinical endocrinology and metabolism |
| container_volume | 86 |
| creator | Blanco, Montserrat López, Miguel GarcÍa-Caballero, Tomás Gallego, RosalÍa Vázquez-Boquete, Ángel Morel, Gerard SeñarÍs, Rosa Casanueva, Felipe Diéguez, Carlos Beiras, Andrés |
| description | Orexins-A and -B are hypothalamic peptides derived from a precursor
called prepro-orexin and relationated with the stimulation of food
intake. They act on G protein receptors named orexin receptor 1
(OX1R) and orexin receptor 2 (OX2R),
respectively. In the present study, we used RT-PCR and
immunohistochemical techniques to detect the presence of
OX1R and OX2R in human pituitary. A band of the
expected size for both OX1R and OX2R was shown
in human pituitary by RT-PCR. The cellular localization of
OX1R and OX2R was carried out using
histological techniques. By consecutive sections we demonstrated that
OX1R was present in acidophil, diffusely distributed cells,
which represent the half of the total adenohypophysis cell population.
As was expected, these cells were shown to coexpress GH.
OX2R was found in the pars intermedia and in clusters of
basophil cells of the anterior pituitary, which coexpress ACTH. These
results were confirmed by double immunofluorescence techniques. We also
found focal positivity in axon terminals of neurohypophysis, more
intense for OX2R than for OX1R. In conclusion,
these results demonstrated for the first time that OX1R and
OX2R were expressed by somatotrope and corticotrope cells,
respectively. |
| doi_str_mv | 10.1210/jcem.86.7.7433 |
| format | article |
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called prepro-orexin and relationated with the stimulation of food
intake. They act on G protein receptors named orexin receptor 1
(OX1R) and orexin receptor 2 (OX2R),
respectively. In the present study, we used RT-PCR and
immunohistochemical techniques to detect the presence of
OX1R and OX2R in human pituitary. A band of the
expected size for both OX1R and OX2R was shown
in human pituitary by RT-PCR. The cellular localization of
OX1R and OX2R was carried out using
histological techniques. By consecutive sections we demonstrated that
OX1R was present in acidophil, diffusely distributed cells,
which represent the half of the total adenohypophysis cell population.
As was expected, these cells were shown to coexpress GH.
OX2R was found in the pars intermedia and in clusters of
basophil cells of the anterior pituitary, which coexpress ACTH. These
results were confirmed by double immunofluorescence techniques. We also
found focal positivity in axon terminals of neurohypophysis, more
intense for OX2R than for OX1R. In conclusion,
these results demonstrated for the first time that OX1R and
OX2R were expressed by somatotrope and corticotrope cells,
respectively.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jcem.86.7.7433</identifier><language>eng</language><publisher>Endocrine Society</publisher><ispartof>The journal of clinical endocrinology and metabolism, 2001-07, Vol.86 (7), p.3444-3447</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2363-595a32478dabd83eb271df25ca0eed301303a74e8d56e177f939b8ac2071bc1e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Blanco, Montserrat</creatorcontrib><creatorcontrib>López, Miguel</creatorcontrib><creatorcontrib>GarcÍa-Caballero, Tomás</creatorcontrib><creatorcontrib>Gallego, RosalÍa</creatorcontrib><creatorcontrib>Vázquez-Boquete, Ángel</creatorcontrib><creatorcontrib>Morel, Gerard</creatorcontrib><creatorcontrib>SeñarÍs, Rosa</creatorcontrib><creatorcontrib>Casanueva, Felipe</creatorcontrib><creatorcontrib>Diéguez, Carlos</creatorcontrib><creatorcontrib>Beiras, Andrés</creatorcontrib><title>Cellular Localization of Orexin Receptors in Human Pituitary</title><title>The journal of clinical endocrinology and metabolism</title><description>Orexins-A and -B are hypothalamic peptides derived from a precursor
called prepro-orexin and relationated with the stimulation of food
intake. They act on G protein receptors named orexin receptor 1
(OX1R) and orexin receptor 2 (OX2R),
respectively. In the present study, we used RT-PCR and
immunohistochemical techniques to detect the presence of
OX1R and OX2R in human pituitary. A band of the
expected size for both OX1R and OX2R was shown
in human pituitary by RT-PCR. The cellular localization of
OX1R and OX2R was carried out using
histological techniques. By consecutive sections we demonstrated that
OX1R was present in acidophil, diffusely distributed cells,
which represent the half of the total adenohypophysis cell population.
As was expected, these cells were shown to coexpress GH.
OX2R was found in the pars intermedia and in clusters of
basophil cells of the anterior pituitary, which coexpress ACTH. These
results were confirmed by double immunofluorescence techniques. We also
found focal positivity in axon terminals of neurohypophysis, more
intense for OX2R than for OX1R. In conclusion,
these results demonstrated for the first time that OX1R and
OX2R were expressed by somatotrope and corticotrope cells,
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called prepro-orexin and relationated with the stimulation of food
intake. They act on G protein receptors named orexin receptor 1
(OX1R) and orexin receptor 2 (OX2R),
respectively. In the present study, we used RT-PCR and
immunohistochemical techniques to detect the presence of
OX1R and OX2R in human pituitary. A band of the
expected size for both OX1R and OX2R was shown
in human pituitary by RT-PCR. The cellular localization of
OX1R and OX2R was carried out using
histological techniques. By consecutive sections we demonstrated that
OX1R was present in acidophil, diffusely distributed cells,
which represent the half of the total adenohypophysis cell population.
As was expected, these cells were shown to coexpress GH.
OX2R was found in the pars intermedia and in clusters of
basophil cells of the anterior pituitary, which coexpress ACTH. These
results were confirmed by double immunofluorescence techniques. We also
found focal positivity in axon terminals of neurohypophysis, more
intense for OX2R than for OX1R. In conclusion,
these results demonstrated for the first time that OX1R and
OX2R were expressed by somatotrope and corticotrope cells,
respectively.</abstract><pub>Endocrine Society</pub><doi>10.1210/jcem.86.7.7433</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The journal of clinical endocrinology and metabolism, 2001-07, Vol.86 (7), p.3444-3447 |
| issn | 0021-972X 1945-7197 |
| language | eng |
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| source | Oxford Journals Online |
| title | Cellular Localization of Orexin Receptors in Human Pituitary |
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