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Silymarin inhibits melanin synthesis in melanocyte cells
Objectives The aim was to search for inhibitors of melanogenesis from natural resources. Methods The inhibitory effect of silymarin on melanogenesis in a spontaneously immortalized mouse melanocyte cell line, Mel‐Ab, was studied. Key findings Silymarin significantly prevented melanin production in a...
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| Published in: | Journal of pharmacy and pharmacology 2009-05, Vol.61 (5), p.663-667 |
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| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c2994-e1120e0e22471bbb5cdd3ceac0bf47d6cdfed888c710a41881f8325743beecb03 |
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| cites | cdi_FETCH-LOGICAL-c2994-e1120e0e22471bbb5cdd3ceac0bf47d6cdfed888c710a41881f8325743beecb03 |
| container_end_page | 667 |
| container_issue | 5 |
| container_start_page | 663 |
| container_title | Journal of pharmacy and pharmacology |
| container_volume | 61 |
| creator | Choo, Soo-Jin Ryoo, In-Ja Kim, Young-Hee Xu, Guang-Hwa Kim, Won-Gon Kim, Ki-Ho Moon, Seong-Joon Son, Eui-Dong Bae, KiHwan Yoo, Ick-Dong |
| description | Objectives The aim was to search for inhibitors of melanogenesis from natural resources.
Methods The inhibitory effect of silymarin on melanogenesis in a spontaneously immortalized mouse melanocyte cell line, Mel‐Ab, was studied.
Key findings Silymarin significantly prevented melanin production in a dose‐dependent manner with an IC50 value (concentration producing 50% maximal inhibition) of 28.2 μg/ml, without effects on cell viability. Also, silymarin inhibited l‐DOPA oxidation activity of tyrosinase, the rate‐limiting melanogenic enzyme, in cell based‐systems but it did not directly affect cell‐free tyrosinase activity. Furthermore, Western blot analysis indicated that silymarin decreased the expression of tyrosinase protein.
Conclusions This study suggests that the depigmenting effect of silymarin might be attributable to inhibition of tyrosinase expression and that silymarin may be useful as a natural skin‐lightening agent. |
| doi_str_mv | 10.1211/jpp.61.05.0016 |
| format | article |
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Methods The inhibitory effect of silymarin on melanogenesis in a spontaneously immortalized mouse melanocyte cell line, Mel‐Ab, was studied.
Key findings Silymarin significantly prevented melanin production in a dose‐dependent manner with an IC50 value (concentration producing 50% maximal inhibition) of 28.2 μg/ml, without effects on cell viability. Also, silymarin inhibited l‐DOPA oxidation activity of tyrosinase, the rate‐limiting melanogenic enzyme, in cell based‐systems but it did not directly affect cell‐free tyrosinase activity. Furthermore, Western blot analysis indicated that silymarin decreased the expression of tyrosinase protein.
Conclusions This study suggests that the depigmenting effect of silymarin might be attributable to inhibition of tyrosinase expression and that silymarin may be useful as a natural skin‐lightening agent.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1211/jpp.61.05.0016</identifier><identifier>PMID: 19406006</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Antioxidants - pharmacology ; Blotting, Western ; Cell Line ; Cell Survival - drug effects ; Levodopa - metabolism ; Melanins - antagonists & inhibitors ; Melanins - biosynthesis ; Melanocytes - drug effects ; Melanocytes - metabolism ; melanogenesis ; Mice ; Monophenol Monooxygenase - antagonists & inhibitors ; Monophenol Monooxygenase - biosynthesis ; silymarin ; Silymarin - pharmacology ; Skin Pigmentation - drug effects ; skin-lightening ; tyrosinase activity</subject><ispartof>Journal of pharmacy and pharmacology, 2009-05, Vol.61 (5), p.663-667</ispartof><rights>2009 Royal Pharmaceutical Society of Great Britain</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2994-e1120e0e22471bbb5cdd3ceac0bf47d6cdfed888c710a41881f8325743beecb03</citedby><cites>FETCH-LOGICAL-c2994-e1120e0e22471bbb5cdd3ceac0bf47d6cdfed888c710a41881f8325743beecb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19406006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choo, Soo-Jin</creatorcontrib><creatorcontrib>Ryoo, In-Ja</creatorcontrib><creatorcontrib>Kim, Young-Hee</creatorcontrib><creatorcontrib>Xu, Guang-Hwa</creatorcontrib><creatorcontrib>Kim, Won-Gon</creatorcontrib><creatorcontrib>Kim, Ki-Ho</creatorcontrib><creatorcontrib>Moon, Seong-Joon</creatorcontrib><creatorcontrib>Son, Eui-Dong</creatorcontrib><creatorcontrib>Bae, KiHwan</creatorcontrib><creatorcontrib>Yoo, Ick-Dong</creatorcontrib><title>Silymarin inhibits melanin synthesis in melanocyte cells</title><title>Journal of pharmacy and pharmacology</title><addtitle>J Pharm Pharmacol</addtitle><description>Objectives The aim was to search for inhibitors of melanogenesis from natural resources.
Methods The inhibitory effect of silymarin on melanogenesis in a spontaneously immortalized mouse melanocyte cell line, Mel‐Ab, was studied.
Key findings Silymarin significantly prevented melanin production in a dose‐dependent manner with an IC50 value (concentration producing 50% maximal inhibition) of 28.2 μg/ml, without effects on cell viability. Also, silymarin inhibited l‐DOPA oxidation activity of tyrosinase, the rate‐limiting melanogenic enzyme, in cell based‐systems but it did not directly affect cell‐free tyrosinase activity. Furthermore, Western blot analysis indicated that silymarin decreased the expression of tyrosinase protein.
Conclusions This study suggests that the depigmenting effect of silymarin might be attributable to inhibition of tyrosinase expression and that silymarin may be useful as a natural skin‐lightening agent.</description><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Levodopa - metabolism</subject><subject>Melanins - antagonists & inhibitors</subject><subject>Melanins - biosynthesis</subject><subject>Melanocytes - drug effects</subject><subject>Melanocytes - metabolism</subject><subject>melanogenesis</subject><subject>Mice</subject><subject>Monophenol Monooxygenase - antagonists & inhibitors</subject><subject>Monophenol Monooxygenase - biosynthesis</subject><subject>silymarin</subject><subject>Silymarin - pharmacology</subject><subject>Skin Pigmentation - drug effects</subject><subject>skin-lightening</subject><subject>tyrosinase activity</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkF9LwzAUxYMobk5ffZR9gdZ7kzTJHmXoqow5_IPgS2jSlGV23Wgq2m9vR4c--nTh3PM73HsIuUSIkSJer3e7WGAMSQyA4ogMKXAaSUzUMRkCUBqxRLIBOQthDQBSCHFKBjjhIADEkKhnX7abrPbV2Fcrb3wTxhtXZlUnhLZqVi740K16cWvbxo2tK8twTk6KrAzu4jBH5PXu9mWaRvPH2f30Zh5ZOpnwyCFScOAo5RKNMYnNc2ZdZsEUXObC5oXLlVJWImQclcJCMZpIzoxz1gAbkbjPtfU2hNoVelf77uBWI-h9BbqrQAvUkOh9BR1w1QO7T7Nx-Z_98HNnYL3hy5eu_SdOPyzTJeO8o6Ke8qFx379UVn9oIZlM9Ntipp_S97tFqqgG9gMREHdG</recordid><startdate>200905</startdate><enddate>200905</enddate><creator>Choo, Soo-Jin</creator><creator>Ryoo, In-Ja</creator><creator>Kim, Young-Hee</creator><creator>Xu, Guang-Hwa</creator><creator>Kim, Won-Gon</creator><creator>Kim, Ki-Ho</creator><creator>Moon, Seong-Joon</creator><creator>Son, Eui-Dong</creator><creator>Bae, KiHwan</creator><creator>Yoo, Ick-Dong</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200905</creationdate><title>Silymarin inhibits melanin synthesis in melanocyte cells</title><author>Choo, Soo-Jin ; Ryoo, In-Ja ; Kim, Young-Hee ; Xu, Guang-Hwa ; Kim, Won-Gon ; Kim, Ki-Ho ; Moon, Seong-Joon ; Son, Eui-Dong ; Bae, KiHwan ; Yoo, Ick-Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2994-e1120e0e22471bbb5cdd3ceac0bf47d6cdfed888c710a41881f8325743beecb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Levodopa - metabolism</topic><topic>Melanins - antagonists & inhibitors</topic><topic>Melanins - biosynthesis</topic><topic>Melanocytes - drug effects</topic><topic>Melanocytes - metabolism</topic><topic>melanogenesis</topic><topic>Mice</topic><topic>Monophenol Monooxygenase - antagonists & inhibitors</topic><topic>Monophenol Monooxygenase - biosynthesis</topic><topic>silymarin</topic><topic>Silymarin - pharmacology</topic><topic>Skin Pigmentation - drug effects</topic><topic>skin-lightening</topic><topic>tyrosinase activity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choo, Soo-Jin</creatorcontrib><creatorcontrib>Ryoo, In-Ja</creatorcontrib><creatorcontrib>Kim, Young-Hee</creatorcontrib><creatorcontrib>Xu, Guang-Hwa</creatorcontrib><creatorcontrib>Kim, Won-Gon</creatorcontrib><creatorcontrib>Kim, Ki-Ho</creatorcontrib><creatorcontrib>Moon, Seong-Joon</creatorcontrib><creatorcontrib>Son, Eui-Dong</creatorcontrib><creatorcontrib>Bae, KiHwan</creatorcontrib><creatorcontrib>Yoo, Ick-Dong</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choo, Soo-Jin</au><au>Ryoo, In-Ja</au><au>Kim, Young-Hee</au><au>Xu, Guang-Hwa</au><au>Kim, Won-Gon</au><au>Kim, Ki-Ho</au><au>Moon, Seong-Joon</au><au>Son, Eui-Dong</au><au>Bae, KiHwan</au><au>Yoo, Ick-Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silymarin inhibits melanin synthesis in melanocyte cells</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><addtitle>J Pharm Pharmacol</addtitle><date>2009-05</date><risdate>2009</risdate><volume>61</volume><issue>5</issue><spage>663</spage><epage>667</epage><pages>663-667</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><abstract>Objectives The aim was to search for inhibitors of melanogenesis from natural resources.
Methods The inhibitory effect of silymarin on melanogenesis in a spontaneously immortalized mouse melanocyte cell line, Mel‐Ab, was studied.
Key findings Silymarin significantly prevented melanin production in a dose‐dependent manner with an IC50 value (concentration producing 50% maximal inhibition) of 28.2 μg/ml, without effects on cell viability. Also, silymarin inhibited l‐DOPA oxidation activity of tyrosinase, the rate‐limiting melanogenic enzyme, in cell based‐systems but it did not directly affect cell‐free tyrosinase activity. Furthermore, Western blot analysis indicated that silymarin decreased the expression of tyrosinase protein.
Conclusions This study suggests that the depigmenting effect of silymarin might be attributable to inhibition of tyrosinase expression and that silymarin may be useful as a natural skin‐lightening agent.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19406006</pmid><doi>10.1211/jpp.61.05.0016</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of pharmacy and pharmacology, 2009-05, Vol.61 (5), p.663-667 |
| issn | 0022-3573 2042-7158 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Animals Antioxidants - pharmacology Blotting, Western Cell Line Cell Survival - drug effects Levodopa - metabolism Melanins - antagonists & inhibitors Melanins - biosynthesis Melanocytes - drug effects Melanocytes - metabolism melanogenesis Mice Monophenol Monooxygenase - antagonists & inhibitors Monophenol Monooxygenase - biosynthesis silymarin Silymarin - pharmacology Skin Pigmentation - drug effects skin-lightening tyrosinase activity |
| title | Silymarin inhibits melanin synthesis in melanocyte cells |
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