κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA

• Published in: Bulletin of the Chemical Society of Japan 2022-01, Vol.95 (1), p.198-207
• Main Authors: Salahuddin, Bidita, Masud, Mostafa Kamal, Aziz, Shazed, Liu, Chia-Hung, Amiralian, Nasim, Ashok, Aditya, Hossain, S M Azad, Park, Hyeongyu, Wahab, Md Abdul, Amin, Mohammed A, Chari, M Adharvana, Rowan, Alan E, Yamauchi, Yusuke, Hossain, Md Shahriar A, Kaneti, Yusuf Valentino
• Format: Article
• Language: English
• ISSN: 0009-2673; 1348-0634
• DOI: 10.1246/bcsj.20210286

Hydrogel-functionalized surface-based transducers demonstrate fluid-like kinetics, non-fouling properties, and superior biocompatibility. The integration of such properties of three-dimensional (3D) micro- or macrostructure of hydrogels into a mesoporous platform provides a favorable moiety for incorporating biomolecule for adsorption or hybridization with a capture agent. Herein, we report a novel κ-carrageenan hydrogel-coated mesoporous gold (Au) electrode (abbreviated as MPGE/gel) for chronocoulometric (CC) detection of microRNA (miRNA). The κ-carrageenan gel provides a 3D porous network on Au electrode surface to enable higher adsorption of target miRNA for CC interrogation of miRNA in presence of a redox molecule-ruthenium hexaammine (III) chloride ([Ru(NH3)6]3+, RuHex). Magnetically isolated and purified target miR-9-2 is adsorbed onto the MPGE via Au-RNA affinity interaction through the porous 3D network of the gel followed by the CC detection. The enhanced miRNA adsorption and electrocatalytic activity of MPGE/gel provide attomolar (50 aM) level of detection of miRNA with a dynamic range from 100 pM to 10 aM. The good reproducibility (% RSD ≤ 5%, for n = 3) and high specificity of the developed biosensor demonstrates its excellent translational potential toward developing precisely controlled sensing devices for current clinical needs.