A Mechanistic Study for 4A1 Antibody-Catalyzed Hydrolysis : Detection of Acyl Intermediate

Recent study identified a hydrolytic abzyme 4A1, that has a significant rate enhancement in hydrolysis of carbonate substrates differing from the inducing hapten in structure around the scissile bond. Kinetic investigation suggested the existence of other affinity elements for potential substrate si...

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Bibliographic Details
Published in:Chemistry letters 1997-12, Vol.26 (12), p.1225-1226
Main Authors: Wada, Yumiko, Sudo, Yukio, Ono, Mitsunori
Format: Article
Language:English
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Summary:Recent study identified a hydrolytic abzyme 4A1, that has a significant rate enhancement in hydrolysis of carbonate substrates differing from the inducing hapten in structure around the scissile bond. Kinetic investigation suggested the existence of other affinity elements for potential substrate side chains. This paper presents further study of 4A1-catalyzed hydrolysis based on detection of an acyl intermediate in the hydrolytic reaction.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.1997.1225