Inhibitory Effect of Norditerpenes on LPS-Induced TNF-α Production from the Okinawan Soft Coral, Sinularia sp

An ethanol (EtOH) extract of the soft coral (Sinularia sp.), collected in Okinawa, demonstrated a potent inhibitory effect on lipopolysaccharide (LPS)-induced tumor necrosis factor-α (TNF-α) production by murine macrophage-like cells (RAW264.7). The activity-guided purification of the EtOH extract r...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2003, Vol.26(3), pp.380-382
Main Authors: Takaki, Hiroko, Koganemaru, Ryoji, Iwakawa, You, Higuchi, Ryuichi, Miyamoto, Tomofumi
Format: Article
Language:English
Subjects:
Animals
Anthozoa - chemistry
Biological and medical sciences
Cell Line
Chromatography, High Pressure Liquid
Diterpenes - isolation & purification
Diterpenes - pharmacology
Dose-Response Relationship, Drug
Drug Interactions
Ethanol - pharmacology
Glucocorticoids - pharmacology
Human Growth Hormone - chemistry
Human Growth Hormone - classification
Human Growth Hormone - isolation & purification
Human Growth Hormone - pharmacology
Lipopolysaccharides - pharmacology
Macrophages - drug effects
Macrophages - metabolism
Medical sciences
Mice
nitric oxide (NO)
Nitric Oxide - metabolism
norcembrenolide
norditerpene
Prednisolone - pharmacology
RAW264.7
sinuleptolide
Tumor Necrosis Factor-alpha - antagonists & inhibitors
Tumor Necrosis Factor-alpha - biosynthesis
tumor necrosis factor-α (TNF-α)
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Summary:An ethanol (EtOH) extract of the soft coral (Sinularia sp.), collected in Okinawa, demonstrated a potent inhibitory effect on lipopolysaccharide (LPS)-induced tumor necrosis factor-α (TNF-α) production by murine macrophage-like cells (RAW264.7). The activity-guided purification of the EtOH extract resulted in the isolation of two norditerpenes, norcembrenolide (1) and sinuleptolide (2). These structures were identified from the spectroscopic data. Norcembrenolide (1) and sinuleptolide (2) inhibited TNF-α production in a dose-dependent manner, and showed a more potent effect than prednisolone at the concentration of 33 μg/ml. They also exhibited an inhibitory effect on nitric oxide (NO) production not influenced by cytotoxicity.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.26.380