Serum Levels of S-Glutathionylated Proteins as a Risk-Marker for Arteriosclerosis Obliterans

Background Oxidative stress plays a role in the development of chronic peripheral arterial disease (PAD) because under these conditions redox regulation is impaired, inducing the S-glutathionylation of proteins. A method of estimating the levels of S-glutathionylated proteins has been developed usin...

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Bibliographic Details
Published in:Circulation Journal 2007, Vol.71(1), pp.100-105
Main Authors: Nonaka, Kazuki, Kume, Noriaki, Urata, Yoshishige, Seto, Shinji, Kohno, Takaaki, Honda, Sumihisa, Ikeda, Soji, Muroya, Takahiro, Ikeda, Yosihiko, Ihara, Yoshito, Kita, Toru, Kondo, Takahito
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Annexin A2 - blood
Arteriosclerosis obliterans
Arteriosclerosis Obliterans - blood
Arteriosclerosis Obliterans - etiology
Arteriosclerosis Obliterans - physiopathology
Biomarkers - blood
Carbonic Anhydrase III - blood
Case-Control Studies
Female
Glutathione - metabolism
Glutathione Transferase - metabolism
Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) - blood
Humans
Male
Middle Aged
Oxidation-Reduction
Oxidative stress
Oxidative Stress - physiology
Protein Kinase C - blood
Risk Factors
S-glutathionylation
Sensitivity and Specificity
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Summary:Background Oxidative stress plays a role in the development of chronic peripheral arterial disease (PAD) because under these conditions redox regulation is impaired, inducing the S-glutathionylation of proteins. A method of estimating the levels of S-glutathionylated proteins has been developed using biotinylated glutathione S-transferase, which allows the study of their crucial role in the oxidative stress-related progression of PAD. Methods and Results The serum levels of S-glutathionylated proteins were examined in 41 patients with arteriosclerosis obliterans (ASO) and 38 age-matched non-ASO patients using biotinylated glutathione S-transferase. The levels were higher in the patients with ASO, even early on, and positively correlated with the ankle/brachial index. In vitro, the levels of S-glutathionylated proteins were reduced in the presence of glutathione and glutaredoxin. Conclusions Serum levels of S-glutathionylated proteins are a sensitive risk-marker for ASO at an early stage. (Circ J 2007; 71: 100 - 105)
ISSN:1346-9843
1347-4820
DOI:10.1253/circj.71.100