Involvement of N-Type Voltage-Activated Ca2+ Channels in the Release of Endogenous Noradrenaline from the Isolated Vascularly Perfused Rat Stomach

We characterized the voltage-activated Ca2+ channels involved in noradrenaline (NA) release from gastric sympathetic neurons using isolated, vascularly perfused rat stomach. The evoked NA release by electrical stimulation of periarterial nerves was abolished by calcium removal from the perfusion med...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1998, Vol.78(1), pp.75-77
Main Authors: Kunihiko, Yokotani, Yasunobu, Okuma, Yoshitsugu, Osumi
Format: Article
Language:English
Subjects:
Animals
Cadmium - pharmacology
Calcium Channel Blockers - pharmacology
Calcium Channels - drug effects
Calcium Channels - physiology
Dose-Response Relationship, Drug
Electric Stimulation
Gastric Mucosa - metabolism
In Vitro Techniques
Male
N-type Ca2+ channel
Noradrenaline release
Norepinephrine - metabolism
omega-Agatoxin IVA
omega-Conotoxin GVIA
omega-Conotoxins
Peptides - pharmacology
Perfusion
Rat stomach
Rats
Rats, Wistar
Spider Venoms - pharmacology
Stomach - drug effects
Stomach - innervation
Sympathetic Nervous System - drug effects
Sympathetic Nervous System - physiology
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Summary:We characterized the voltage-activated Ca2+ channels involved in noradrenaline (NA) release from gastric sympathetic neurons using isolated, vascularly perfused rat stomach. The evoked NA release by electrical stimulation of periarterial nerves was abolished by calcium removal from the perfusion medium and by cadmium. ω-Conotoxin GVIA (N-type Ca2+-channel blocker) effectively and ω-conotoxin MVIIC (N/P/Q-type blocker) slightly inhibited the evoked NA, while ω-agatoxin IVA (P-type blocker) had no effect. These results suggest that ω-conotoxin GVIA and ω-conotoxin MVIIC-sensitive N-type Ca2+ channels are involved in NA release from the rat gastric sympathetic nerve terminals.
ISSN:0021-5198
1347-3506
DOI:10.1254/jjp.78.75