PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies...

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Bibliographic Details
Published in:Laboratory animals (London) 2001-04, Vol.35 (2), p.157-162
Main Authors: Benavides, F, Glasscock, E, Coghlan, L G, Stern, M C, Weiss, D A, Conti, C J
Format: Article
Language:English
Subjects:
Animals
DNA - chemistry
DNA - genetics
DNA - isolation & purification
Female
Genetic Markers - genetics
Mice
Mice, Inbred SENCAR - classification
Mice, Inbred SENCAR - genetics
Microsatellite Repeats - genetics
Polymerase Chain Reaction - veterinary
Polymorphism, Genetic - genetics
Quality Control
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Summary:Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.
ISSN:0023-6772
1758-1117
DOI:10.1258/0023677011911534