The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos

The aim of this study was to investigate if reconstructed felid embryos obtained by intraspecies or intergeneric cloning can develop in vitro. Fibroblast cells (f) from a domestic cat (DCf), marbled cat (MCf) and bovine (Bf) were used as donor cells, and oocytes (o) from domestic cats (DCo) and bovi...

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Published in:Journal of Reproduction and Development 2011, Vol.57(3), pp.385-392
Main Authors: IMSOONTHORNRUKSA, Sumeth, LORTHONGPANICH, Chanchao, SANGMALEE, Anawat, SRIRATTANA, Kanokwan, LAOWTAMMATHRON, Chuti, TUNWATTANA, Wanchai, SOMSA, Wachiravit, KETUDAT-CAIRNS, Mariena, NAGAI, Takashi, PARNPAI, Rangsun
Format: Article
Language:English
Subjects:
Bovine cytoplast
Development
Felid embryos
Intergeneric cloning
Marbled cat
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Summary:The aim of this study was to investigate if reconstructed felid embryos obtained by intraspecies or intergeneric cloning can develop in vitro. Fibroblast cells (f) from a domestic cat (DCf), marbled cat (MCf) and bovine (Bf) were used as donor cells, and oocytes (o) from domestic cats (DCo) and bovine (Bo) were used as recipient cytoplasts. There were two intraspecies (donor cell + recipient cytoplast: DCf + DCo and Bf + Bo) and three intergeneric (MCf + DCo, DCf + Bo and MCf + Bo) cloning groups in the study. In Experiment 1, the effects of manipulation media, modified TCM-199 (199H) or Emcare holding medium (EHM), on in vitro development of DCf + DCo embryos were investigated. The blastocyst formation rate (BFR) of the embryos manipulated in EHM (33.3%) was higher (P
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.10-108H