In-straw Cryoprotectant Dilution for Bovine Embryos Vitrified Using Cryotop

The aim of this study was to develop an in-straw dilution method suitable for 1-step bovine embryo transfer of vitrified embryos using the Cryotop vitrification-straw dilution (CVSD) method. The development of embryos vitrified using the CVSD method was compared with those of embryos cryopreserved u...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2011, Vol.57(4), pp.437-443
Main Authors: INABA, Yasushi, AIKAWA, Yoshio, HIRAI, Tomokazu, HASHIYADA, Yutaka, YAMANOUCHI, Tadayuki, MISUMI, Koji, OHTAKE, Masaki, SOMFAI, Tamas, KOBAYASHI, Shuji, SAITO, Norio, MATOBA, Satoko, KONISHI, Kazuyuki, IMAI, Kei
Format: Article
Language:English
Subjects:
Bovine
Conception rate
Cryotop
Embryo transfer
Vitrification
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Summary:The aim of this study was to develop an in-straw dilution method suitable for 1-step bovine embryo transfer of vitrified embryos using the Cryotop vitrification-straw dilution (CVSD) method. The development of embryos vitrified using the CVSD method was compared with those of embryos cryopreserved using in-straw vitrification-dilution (ISVD) and conventional slow freezing, outside dilution of straw (SFODS) methods. In Experiment 1, in vitro-produced (IVP) embryos cryopreserved using the CVSD method were diluted, warmed and exposed to the dilution solution at various times. When vitrified IVP embryos were exposed to the dilution solution for 30 min after warming, the rates of embryos developing to the hatched blastocyst stage after 72 h of culture (62.0-72.5%) were significantly lower (P
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.10-154M