Sperm Penetration Assay as an Indicator of Bull Fertility

To predict the fertility of frozen-thawed bull spermatozoa, a sperm penetration assay (SPA) using zona-free hamster oocytes was optimized, and the assay results were compared with data from field fertility expressed as the non-return rate (NRR). To increase sperm penetration, the spermatozoa were pr...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2012, Vol.58(4), pp.461-466
Main Authors: PARK, Yoo-Jin, MOHAMED, El-Sayed A., OH, Shin-Ae, YOON, Sung-Jae, KWON, Woo-Sung, KIM, Heung-Ruil, LEE, Myeung-Sik, LEE, Kichoon, PANG, Myung-Geol
Format: Article
Language:English
Subjects:
Bull
Male fertility
Prediction
Sperm
Sperm penetration assay
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Summary:To predict the fertility of frozen-thawed bull spermatozoa, a sperm penetration assay (SPA) using zona-free hamster oocytes was optimized, and the assay results were compared with data from field fertility expressed as the non-return rate (NRR). To increase sperm penetration, the spermatozoa were pre-incubated and coincubated with oocytes in media containing various concentrations of heparin (0 to 50 μg/ml). Coincubation with 10 μg/ml heparin showed the highest sperm penetration (P
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.11-067H