Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, pos...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2012, Vol.58(2), pp.248-253
Main Authors: TAKEDA, Kumiko, TASAI, Mariko, IWAMOTO, Masaki, OE, Mika, CHIKUNI, Koichi, NAKAMURA, Yoshiaki, TAGAMI, Takahiro, NIRASAWA, Keijiro, HANADA, Hirofumi, PINKERT, Carl A., ONISHI, Akira
Format: Article
Language:English
Subjects:
2-D DIGE
Liver
Mitochondrial protein
Pig
Somatic cell nuclear transfer
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Summary:Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, post SCNT, are largely unknown. In the present study, alterations in mitochondrial protein levels were investigated in adult SCNT pigs produced by microinjection of Meishan pig fetus fibroblast cells into enucleated matured oocytes (maternal Landrace genetic background). Mitochondrial fractions were prepared from liver samples by mechanical homogenization and differential centrifugation. Liver mitochondria were then subjected to two-dimensional difference gel electrophoresis (2-D DIGE). Protein expression changes were confirmed with a volume ratio greater than 2 fold (P
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.11-074A