Abnormal accumulation of luteal bodies in ovaries of the senescence accelerated mouse (SAM)

The senescence accelerated mouse prone (SAMP) mice with a shortened life span show accelerated changes in many of the signs of aging and have a shorter reproductive life span than SAM resistant (SAMR) controls. The reproductive senescence of SAMP is more accelerated than that of SAMR. In the present...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Reproduction and Development 2001, Vol.47(3), pp.153-164
Main Authors: Kiso, M. (Kyoto Univ. (Japan)), Manabe, N, Komatsu, K, Nisioka, N, Nakai Sugimoto, N, Miyamoto, H
Format: Article
Language:English
Subjects:
APOPTOSIS
ATROPHY
CORPUS LUTEUM
Luteal body
MICE
Nitric oxide synthase
OVARIES
Ovary
SENESCENCE
Senescence accelerated mouse (SAM)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The senescence accelerated mouse prone (SAMP) mice with a shortened life span show accelerated changes in many of the signs of aging and have a shorter reproductive life span than SAM resistant (SAMR) controls. The reproductive senescence of SAMP is more accelerated than that of SAMR. In the present study, we found abnormal accumulation of luteal bodies (LBs) only in the ovaries of SAMP mice and, we examined the mechanism of abnormalities in luteal cell regression in the ovaries of SAMP mice. No significant changes were detected in serum progesterone or 17 beta -estradiol levels during estrus cycle between SAMP and SAMR mice. In abnormally accumulated LBs of SAMP mice, extremely high levels of activity of 20 alpha -hydroxysteroid dehydrogenase, which catalyzes conversion of progesterone to the inactive form, 20 alpha -hydroxyprogesterone were demonstrated histochemically. However, no marked differences in activity of 17 beta -hydroxysteroid dehydrogenase were detected histochemically. Moreover, no apoptotic cells were detected in the abnormally accumulated LBs of SAMP mice. Extremely weak immunohistochemical reactivity for endothehal nitric oxide synthase (eNOS) was noted in the abnormally accumulated LBs of SAMP mice, but no differences were seen in inducible NOS (iNOS). No marked differences in expression of eNOS- or iNOS-mRNA were seen during the estrus cycle in the ovaries of SAMP mice. However high levels of expression of eNOS-mRNA at estrus stage and of iNOS-mRNA at estrus and metestrus stages were noted in the ovaries of SAMR mice. SAMP mice may be a useful model in which to examine the mechanism of regression of LBs in mammalian ovaries.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.47.153