Bovine nucleus transplantation by intracytoplasmic injection

This study investigated bovine somatic nucleus transplantation (NT) using an intracytoplasmic injection system in which cumulus cell nuclei were injected into enucleated oocytes by a piezo micromanipulator. Activation of NT embryos was carried out by (1) simultaneous injection of 2-4 pl of inositol...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Reproduction and Development 2002, Vol.48(6), pp.619-626
Main Authors: Ushijima, H. (Chiba-ken. Livestock Research Center, Yachimata (Japan)), Ishida, K, Nagashima, H
Format: Article
Language:English
Subjects:
CATTLE
CYTOPLASM
In vitro-produced bovine embryo
Intracytoplasmic injection
NUCLEUS
Nucleus transplantation
Parthenogenetic activation
TRANSPLANTATION
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study investigated bovine somatic nucleus transplantation (NT) using an intracytoplasmic injection system in which cumulus cell nuclei were injected into enucleated oocytes by a piezo micromanipulator. Activation of NT embryos was carried out by (1) simultaneous injection of 2-4 pl of inositol 1,4,5 tri-phosphate (IP3) and a donor nucleus into a recipient cytoplasm (IP method), (2) IP method followed by 1.9 mM 6-dimethylaminopurine (DM) treatment for 3.5 h (IP+DM method), or (3) activated after NT by an ionomycin treatment (IA) followed by DM treatment (IA+DM method). We found the frequency of remodeling nuclei and development into blastocyst stage of NT embryos to be: IP, 44% and 9%; IP+DM, 58% and 29%; and IA+DM, 61% and 27%. These results indicate that IP3 injection into the intracytoplasm followed by DM treatment produces a similar bovine oocyte parthenogenetic activation as IA+DM activation treatment, and that intracytoplasmic nucleus injection is an effective method for bovine somatic NT.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.48.619