The effect of soaking heat-polymerized acrylic resin denture base in avocado seed extract ( Persea americana Mill.) on the inhibition of denture-plaque microorganisms biofilm growth   [version 1; peer review: awaiting peer review]

Background Heat polymerized acrylic (HPA) resins are known to have high porosity that contributes to increased surface roughness and microcrack formation in stress areas. This facilitates the attachment and growth of polymicrobial biofilms contributing to increased antimicrobial resistance. Many res...

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Published in:F1000 research 2024, Vol.13, p.933
Main Authors: Angela, Thalia, Wahyuni, Siti, Halim, Susanna
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Halim, Susanna
description Background Heat polymerized acrylic (HPA) resins are known to have high porosity that contributes to increased surface roughness and microcrack formation in stress areas. This facilitates the attachment and growth of polymicrobial biofilms contributing to increased antimicrobial resistance. Many research had been carried out on avocado seeds, but no research that studies the effect of avocado seeds on denture-plaque microorganism biofilm on HPA resin has been found. Methods This study used 144 samples (n=144), namely HPA resin discs covered with mono-species and polymicrobial biofilms consisting of Candida albicans, Candida glabrata, Actinomyces odontolyticus, Streptococcus gordonii, and Staphylococcus aureus. The discs were soaked for 8 hours in the 5%, 10%, 15%, 20% avocado seed extract, positive control (alkaline peroxide), and negative control (aquadest). Each disc was shaken with a vortex mixer for 1 minute, and 100 μL was added into 96-well microplates with three times repetition and incubated for 24 hours. The inhibition values were determined from the percentage inhibition value formula which required absorption values from a microplate reader (595 nm). Results In this research, it was found that the MBIC 50 of avocado seed extract against the mono-species of C. albicans (5%), C. glabrata (5%), A. odontolyticus (15%), S. gordonii (15%), S. aureus (10%), while against the biofilm was 20%. There was a significant effect of soaking HPA resin in avocado seed extract of 5%, 10%, 15%, 20% on the inhibition of mono-species and polymicrobial biofilms of denture-plaque microorganisms with a value of p
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This facilitates the attachment and growth of polymicrobial biofilms contributing to increased antimicrobial resistance. Many research had been carried out on avocado seeds, but no research that studies the effect of avocado seeds on denture-plaque microorganism biofilm on HPA resin has been found. Methods This study used 144 samples (n=144), namely HPA resin discs covered with mono-species and polymicrobial biofilms consisting of Candida albicans, Candida glabrata, Actinomyces odontolyticus, Streptococcus gordonii, and Staphylococcus aureus. The discs were soaked for 8 hours in the 5%, 10%, 15%, 20% avocado seed extract, positive control (alkaline peroxide), and negative control (aquadest). Each disc was shaken with a vortex mixer for 1 minute, and 100 μL was added into 96-well microplates with three times repetition and incubated for 24 hours. The inhibition values were determined from the percentage inhibition value formula which required absorption values from a microplate reader (595 nm). Results In this research, it was found that the MBIC 50 of avocado seed extract against the mono-species of C. albicans (5%), C. glabrata (5%), A. odontolyticus (15%), S. gordonii (15%), S. aureus (10%), while against the biofilm was 20%. There was a significant effect of soaking HPA resin in avocado seed extract of 5%, 10%, 15%, 20% on the inhibition of mono-species and polymicrobial biofilms of denture-plaque microorganisms with a value of p&lt;0.001 (p&lt;0.05). Conclusion The MBIC 50 of avocado seed extract in polymicrobial biofilm group was higher than that in the mono-species biofilm groups. Although alkaline peroxide showed higher inhibition value than that of the MBIC 50 in polymicrobial biofilm group, 20% avocado seed extract was effective in inhibiting polymicrobial biofilm because it was able to inhibit more than 50% polymicrobial biofilm.</description><identifier>ISSN: 2046-1402</identifier><identifier>EISSN: 2046-1402</identifier><identifier>DOI: 10.12688/f1000research.152800.1</identifier><language>eng</language><ispartof>F1000 research, 2024, Vol.13, p.933</ispartof><rights>Copyright: © 2024 Angela T et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1491-d26aa355b6097616d834f32cbd97d64a3136cb8b9848e3837a168a9c9173ede03</cites><orcidid>0009-0000-3692-545X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Angela, Thalia</creatorcontrib><creatorcontrib>Wahyuni, Siti</creatorcontrib><creatorcontrib>Halim, Susanna</creatorcontrib><title>The effect of soaking heat-polymerized acrylic resin denture base in avocado seed extract ( Persea americana Mill.) on the inhibition of denture-plaque microorganisms biofilm growth   [version 1; peer review: awaiting peer review]</title><title>F1000 research</title><description>Background Heat polymerized acrylic (HPA) resins are known to have high porosity that contributes to increased surface roughness and microcrack formation in stress areas. This facilitates the attachment and growth of polymicrobial biofilms contributing to increased antimicrobial resistance. Many research had been carried out on avocado seeds, but no research that studies the effect of avocado seeds on denture-plaque microorganism biofilm on HPA resin has been found. Methods This study used 144 samples (n=144), namely HPA resin discs covered with mono-species and polymicrobial biofilms consisting of Candida albicans, Candida glabrata, Actinomyces odontolyticus, Streptococcus gordonii, and Staphylococcus aureus. The discs were soaked for 8 hours in the 5%, 10%, 15%, 20% avocado seed extract, positive control (alkaline peroxide), and negative control (aquadest). Each disc was shaken with a vortex mixer for 1 minute, and 100 μL was added into 96-well microplates with three times repetition and incubated for 24 hours. The inhibition values were determined from the percentage inhibition value formula which required absorption values from a microplate reader (595 nm). Results In this research, it was found that the MBIC 50 of avocado seed extract against the mono-species of C. albicans (5%), C. glabrata (5%), A. odontolyticus (15%), S. gordonii (15%), S. aureus (10%), while against the biofilm was 20%. There was a significant effect of soaking HPA resin in avocado seed extract of 5%, 10%, 15%, 20% on the inhibition of mono-species and polymicrobial biofilms of denture-plaque microorganisms with a value of p&lt;0.001 (p&lt;0.05). Conclusion The MBIC 50 of avocado seed extract in polymicrobial biofilm group was higher than that in the mono-species biofilm groups. Although alkaline peroxide showed higher inhibition value than that of the MBIC 50 in polymicrobial biofilm group, 20% avocado seed extract was effective in inhibiting polymicrobial biofilm because it was able to inhibit more than 50% polymicrobial biofilm.</description><issn>2046-1402</issn><issn>2046-1402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkcFO3DAQhqMKpCLKM3SO7SGLHWcdB04VAooEKgc4oSqaOOONSxJv7exul6fhWXiaPgbeLhL0xMme8f9_M9afJJ85m_BMKnVoOGPMUyD0up3waaZYfPqQ7GUslynPWbbz5v4xOQjhV3SwshQyK_aSvzctARlDegRnIDi8t8MMWsIxnbtu3ZO3D9QAar_urIY4yg7Q0DAuPEGNgSDWuHQaGweBopT-jB4j7gtck4-LAW4gGgeEK9t1k6_gBhjbjbG1tR1tLOPoF2Y67_D3gqC32jvnZzjY0AeorTO262Hm3Wpsnx6fHuFuGfEbMz-GOZGPuy0trY4AVxip8Rdvuj8_JbsGu0AHL-d-cnt2enPyPb38cX5x8u0y1TwvedpkElFMp7VkZSG5bJTIjch03ZRFI3MUXEhdq7pUuSKhRIFcKix1yQtBDTGxnxRbblw_BE-mmnvbo19XnFX_Mqv-y6zaZlbx6DzeOg3qRTeuN6rqVfaO-xne6aZT</recordid><startdate>2024</startdate><enddate>2024</enddate><creator>Angela, Thalia</creator><creator>Wahyuni, Siti</creator><creator>Halim, Susanna</creator><scope>C-E</scope><scope>CH4</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0009-0000-3692-545X</orcidid></search><sort><creationdate>2024</creationdate><title>The effect of soaking heat-polymerized acrylic resin denture base in avocado seed extract ( Persea americana Mill.) on the inhibition of denture-plaque microorganisms biofilm growth   [version 1; peer review: awaiting peer review]</title><author>Angela, Thalia ; Wahyuni, Siti ; Halim, Susanna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1491-d26aa355b6097616d834f32cbd97d64a3136cb8b9848e3837a168a9c9173ede03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angela, Thalia</creatorcontrib><creatorcontrib>Wahyuni, Siti</creatorcontrib><creatorcontrib>Halim, Susanna</creatorcontrib><collection>F1000Research</collection><collection>Faculty of 1000</collection><collection>CrossRef</collection><jtitle>F1000 research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angela, Thalia</au><au>Wahyuni, Siti</au><au>Halim, Susanna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of soaking heat-polymerized acrylic resin denture base in avocado seed extract ( Persea americana Mill.) on the inhibition of denture-plaque microorganisms biofilm growth   [version 1; peer review: awaiting peer review]</atitle><jtitle>F1000 research</jtitle><date>2024</date><risdate>2024</risdate><volume>13</volume><spage>933</spage><pages>933-</pages><issn>2046-1402</issn><eissn>2046-1402</eissn><abstract>Background Heat polymerized acrylic (HPA) resins are known to have high porosity that contributes to increased surface roughness and microcrack formation in stress areas. This facilitates the attachment and growth of polymicrobial biofilms contributing to increased antimicrobial resistance. Many research had been carried out on avocado seeds, but no research that studies the effect of avocado seeds on denture-plaque microorganism biofilm on HPA resin has been found. Methods This study used 144 samples (n=144), namely HPA resin discs covered with mono-species and polymicrobial biofilms consisting of Candida albicans, Candida glabrata, Actinomyces odontolyticus, Streptococcus gordonii, and Staphylococcus aureus. The discs were soaked for 8 hours in the 5%, 10%, 15%, 20% avocado seed extract, positive control (alkaline peroxide), and negative control (aquadest). Each disc was shaken with a vortex mixer for 1 minute, and 100 μL was added into 96-well microplates with three times repetition and incubated for 24 hours. The inhibition values were determined from the percentage inhibition value formula which required absorption values from a microplate reader (595 nm). Results In this research, it was found that the MBIC 50 of avocado seed extract against the mono-species of C. albicans (5%), C. glabrata (5%), A. odontolyticus (15%), S. gordonii (15%), S. aureus (10%), while against the biofilm was 20%. There was a significant effect of soaking HPA resin in avocado seed extract of 5%, 10%, 15%, 20% on the inhibition of mono-species and polymicrobial biofilms of denture-plaque microorganisms with a value of p&lt;0.001 (p&lt;0.05). Conclusion The MBIC 50 of avocado seed extract in polymicrobial biofilm group was higher than that in the mono-species biofilm groups. Although alkaline peroxide showed higher inhibition value than that of the MBIC 50 in polymicrobial biofilm group, 20% avocado seed extract was effective in inhibiting polymicrobial biofilm because it was able to inhibit more than 50% polymicrobial biofilm.</abstract><doi>10.12688/f1000research.152800.1</doi><orcidid>https://orcid.org/0009-0000-3692-545X</orcidid><oa>free_for_read</oa></addata></record>
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title The effect of soaking heat-polymerized acrylic resin denture base in avocado seed extract ( Persea americana Mill.) on the inhibition of denture-plaque microorganisms biofilm growth   [version 1; peer review: awaiting peer review]
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