Cytotoxic T cells response with decreased CD4/CD8 ratio during mammary tumors inhibition in rats induced by non-contact electric fields [version 1; peer review: 1 approved, 1 approved with reservations]

• Published in: F1000 research 2021, Vol.10, p.35
• Main Authors: Alamsyah, Firman, Pratiwi, Rarastoeti, Firdausi, Nisrina, Irene Mesak Pello, Jessica, Evi Dwi Nugraheni, Subekti, Ghitha Fadhlurrahman, Ahmad, Nurhidayat, Luthfi, Purwo Taruno, Warsito
• Format: Article
• Language: English
• Subjects: Animals; CD8-Positive T-Lymphocytes; Female; Mammary Neoplasms, Animal - therapy; Mammary Neoplasms, Experimental - chemically induced; Mammary Neoplasms, Experimental - therapy; Rats; Rats, Sprague-Dawley
• ISSN: 2046-1402; 2046-1402
• DOI: 10.12688/f1000research.27952.1

Background: Breast cancer is the most common cancer in women worldwide and is the leading cause of death in women with cancer. One novel therapy used for breast cancer treatment is non-contact electric fields called electro-capacitive cancer therapy (ECCT) with intermediate frequency (100 kHz) and low intensity (18 Vpp). The objective of this study was to examine the effect of ECCT on mammary tumors growth in rats and observing the immune responses that play a role in fighting the tumor. Methods: Female SD rats were used and divided into four groups, namely control (NINT), placebo (NIT), non- therapy (INT), and therapy (IT) groups with 6 biological replicates in each group. Rats in INT and IT groups were treated with 7,12-dimethylbenz[a]anthracene for mammary tumor induction. Only rats in NIT and IT groups were exposed to ECCT individually for 10 hours per day for 21 days. The size of all tumors was measured with a digital caliper. The distributions of PCNA, ErbB2, caspase-3, CD68, CD4 and CD8-positive cells were observed with immunohistochemistry and scoring with ImageJ. Results: The growth rate of mammary tumors in IT group was significantly lower (p