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Role of Glutathione in the Accumulation of Potassium Ions in Escherichia coli under Osmotic Stress
When Escherichia coli cells were stressed by NaCl, cellular levels of glutathione (GSH) increased, and potassium ions (K + ) accumulated. To identify the role of the stress-induced synthesis of GSH in the accumulation of K + , a GSH-deficient mutant of E. coli was used. The mutant grew even in a med...
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| Published in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1992, Vol.56 (4), p.640-643 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| container_end_page | 643 |
| container_issue | 4 |
| container_start_page | 640 |
| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 56 |
| creator | Ohwada, Takuji Sagisaka, Shonosuke |
| description | When Escherichia coli cells were stressed by NaCl, cellular levels of glutathione (GSH) increased, and potassium ions (K
+
) accumulated. To identify the role of the stress-induced synthesis of GSH in the accumulation of K
+
, a GSH-deficient mutant of E. coli was used. The mutant grew even in a medium with a low concentration of K
+
after a lag of about 32 hr. In such cells grown under osmotic stress in this medium, K
+
did not accumulate in the lag phase. In the logarithmic phase, however, intracellular levels of K
+
increased in spite of the lack of intracellular GSH. In a medium with the usual K
+
concentration, K
+
accumulated in a pattern similar to that of the parent strain in both phases. These results showed that GSH participates in the accumulation of K
+
in cells under osmotic stress only in the lag phase in medium with little K
+
. |
| doi_str_mv | 10.1271/bbb.56.640 |
| format | article |
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+
) accumulated. To identify the role of the stress-induced synthesis of GSH in the accumulation of K
+
, a GSH-deficient mutant of E. coli was used. The mutant grew even in a medium with a low concentration of K
+
after a lag of about 32 hr. In such cells grown under osmotic stress in this medium, K
+
did not accumulate in the lag phase. In the logarithmic phase, however, intracellular levels of K
+
increased in spite of the lack of intracellular GSH. In a medium with the usual K
+
concentration, K
+
accumulated in a pattern similar to that of the parent strain in both phases. These results showed that GSH participates in the accumulation of K
+
in cells under osmotic stress only in the lag phase in medium with little K
+
.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.56.640</identifier><identifier>PMID: 27280662</identifier><language>eng</language><publisher>Tokyo: Taylor & Francis</publisher><subject>Action of physical and chemical agents on bacteria ; Bacteriology ; Biological and medical sciences ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Microbiology</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1992, Vol.56 (4), p.640-643</ispartof><rights>Copyright 1992 Taylor and Francis Group LLC 1992</rights><rights>1992 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1992</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5622464$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27280662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohwada, Takuji</creatorcontrib><creatorcontrib>Sagisaka, Shonosuke</creatorcontrib><title>Role of Glutathione in the Accumulation of Potassium Ions in Escherichia coli under Osmotic Stress</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>When Escherichia coli cells were stressed by NaCl, cellular levels of glutathione (GSH) increased, and potassium ions (K
+
) accumulated. To identify the role of the stress-induced synthesis of GSH in the accumulation of K
+
, a GSH-deficient mutant of E. coli was used. The mutant grew even in a medium with a low concentration of K
+
after a lag of about 32 hr. In such cells grown under osmotic stress in this medium, K
+
did not accumulate in the lag phase. In the logarithmic phase, however, intracellular levels of K
+
increased in spite of the lack of intracellular GSH. In a medium with the usual K
+
concentration, K
+
accumulated in a pattern similar to that of the parent strain in both phases. These results showed that GSH participates in the accumulation of K
+
in cells under osmotic stress only in the lag phase in medium with little K
+
.</description><subject>Action of physical and chemical agents on bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNp90VtLHTEQB_BQlHpq-9IPUAIWKYU9zT3ZRxFrBUHp5Tlks1lOJLvRXCh-e3M4Rx-K9Glg-M0Mwx-AjxitMZH42zAMay7WgqE3YIUpk53omTwAK9Rj0SnG8RF4l_MdQq3B8VtwRCRRSAiyAsPPGByME7wMtZiy8XFx0C-wbBw8s7bONZjSmltyG4vJ2dcZXsUlb9VFthuXvN14A20MHtZldAne5DkWb-GvklzO78HhZEJ2H_b1GPz5fvH7_Ed3fXN5dX523VmmcOkcdbZXI3YTowPBgkthKMaG9FTaUVpJOR25ohRPIx3ZwChnzBqBJ4WsdYgegy-7vfcpPlSXi559ti4Es7hYs8ay50oiwnijp_-nQsleINngyT_wLta0tDc0ZqynPVGENfV1p2yKOSc36fvkZ5MeNUZ6G5FuEWkudIuo4U_7lXWY3fhCnzNp4PMemGxNmJJZrM8vjgtCmNge5Tvmlymm2fyNKYy6mMcQ0_MMfeX-ExiMqes</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Ohwada, Takuji</creator><creator>Sagisaka, Shonosuke</creator><general>Taylor & Francis</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Role of Glutathione in the Accumulation of Potassium Ions in Escherichia coli under Osmotic Stress</title><author>Ohwada, Takuji ; Sagisaka, Shonosuke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-e3ec98d1ef43b216576a311a2937cd7c7353d58331fd3d4b43544ca61f80cce03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Action of physical and chemical agents on bacteria</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohwada, Takuji</creatorcontrib><creatorcontrib>Sagisaka, Shonosuke</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohwada, Takuji</au><au>Sagisaka, Shonosuke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Glutathione in the Accumulation of Potassium Ions in Escherichia coli under Osmotic Stress</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1992</date><risdate>1992</risdate><volume>56</volume><issue>4</issue><spage>640</spage><epage>643</epage><pages>640-643</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>When Escherichia coli cells were stressed by NaCl, cellular levels of glutathione (GSH) increased, and potassium ions (K
+
) accumulated. To identify the role of the stress-induced synthesis of GSH in the accumulation of K
+
, a GSH-deficient mutant of E. coli was used. The mutant grew even in a medium with a low concentration of K
+
after a lag of about 32 hr. In such cells grown under osmotic stress in this medium, K
+
did not accumulate in the lag phase. In the logarithmic phase, however, intracellular levels of K
+
increased in spite of the lack of intracellular GSH. In a medium with the usual K
+
concentration, K
+
accumulated in a pattern similar to that of the parent strain in both phases. These results showed that GSH participates in the accumulation of K
+
in cells under osmotic stress only in the lag phase in medium with little K
+
.</abstract><cop>Tokyo</cop><pub>Taylor & Francis</pub><pmid>27280662</pmid><doi>10.1271/bbb.56.640</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 1992, Vol.56 (4), p.640-643 |
| issn | 0916-8451 1347-6947 |
| language | eng |
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| source | EZB Free E-Journals |
| subjects | Action of physical and chemical agents on bacteria Bacteriology Biological and medical sciences Escherichia coli Fundamental and applied biological sciences. Psychology Microbiology |
| title | Role of Glutathione in the Accumulation of Potassium Ions in Escherichia coli under Osmotic Stress |
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