Distribution of α-Toc Stereoisomers in Rats

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1994, Vol.58 (11), p.2000-2003
• Main Authors: Nitta-Kiyose, Chikako, Hayashi, Kazue, Ueda, Tadahiko, Igarashi, Osamu
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.58.2000

The distribution of α-tocopherol (α-Toc) stereoisomers in the tissues of rats fed on diets containing different levels of all-rac-α-tocopheryl acetate was determined by a newly revised HPLC method and compared with that of RRR-α-Toc fed rats. By this method, all-rac-α-Toc in the blood and tissues was completely separated into 2R-isomers (one peak) and 2R-isomers (three peaks). The concentrations of the 2R-isomers of α-Toc in the blood and tissues of the rats in the all-rac-α-tocopheryl acetate group were significantly higher than those of the 2S-isomers. In most tissues, the level of 2S-isomers was in order of SRS> (SSS+SSR)/2 > SRR. In the RRR-α-Toc-fed groups, the concentration of RRR-α-Toc in every tissue depended on the dose of α-Toc. When the concentration of 2R-isomers in the tissues of rats fed on all-rac-α-Toc (100 mg/kg diet) is compared with the concentration of RRR-α-Toc in the tissues of rats fed on RRR-α-Toc (50 mg/kg diet) the concentration of 2R-isomers in every tissue of the former is the same as the RRR-α-Toc concentration of the latter.