Variation of Inhibition Modes by n-Alcohols in Arbutin (Hydroquinone-O-β-D-glucopyranoside) Hydrolysis by β-Glucosidase

When n-alcohols were added to the β-glucosidase-catalyzed hydrolysis of arbutin, the rate was decreased almost linearly with their elevating concentrations. The inhibition was shown in two modes, competitive for higher n-alcohols and noncompetitive for lower n-alcohols than n-butanol. The inhibition...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1995, Vol.59 (7), p.1352-1354, Article 1352
Main Authors: Sakama, Noriko, Tadasa, Koji
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
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Summary:When n-alcohols were added to the β-glucosidase-catalyzed hydrolysis of arbutin, the rate was decreased almost linearly with their elevating concentrations. The inhibition was shown in two modes, competitive for higher n-alcohols and noncompetitive for lower n-alcohols than n-butanol. The inhibition constants (K i ) obtained, moreover, clearly demonstrated classifying the modes in two groups according to physicochemical parameters, log P, Hildebrand solubility parameter (δ), and dielectric constant (ɛ), of n-alcohols. n-Butanol being between two modes of inhibition showed a mixed-type inhibition. The data suggested that the inhibition of n-alcohols for p-glucosidase may be related with their chain length. An imaging model in this case of inhibition is presented.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.59.1352