Cloning and Characterization of Mycovirus Double-stranded RNA from the Plant Pathogenic Fungus, Fusarium solani f. sp. robiniae

A mycovirus (named FusoV) from the phytopathogenic fungus, Fusarium solani f. sp. robiniae SUF704, has two kinds of double-stranded (ds) RNA genomes, designated Ml and M2. The cDNAs were constructed from FusoV genomic dsRNAs. The sequences of Ml and M2 cDNAs comprised 1645 and 1445 bp, respectively....

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1996, Vol.60 (5), p.784-788
Main Authors: Nogawa, Masahiro, Kageyama, Tsutomu, Nakatani, Aiko, Taguchi, Goro, Shimosaka, Makoto, Okazaki, Mitsuo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Autoradiography
Base Sequence
Biological and medical sciences
Biological Evolution
Biotechnology
Capsid - metabolism
capsid protein
cDNA synthesis
Cloning, Molecular
Consensus Sequence
Conserved Sequence
DNA Replication
DNA, Complementary - chemistry
DNA, Complementary - genetics
DNA-Directed RNA Polymerases - chemistry
DNA-Directed RNA Polymerases - genetics
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Fusarium - virology
Genetics
Immunoblotting
in vitro replication
Microbiology
Molecular Sequence Data
Molecular Weight
Nucleic Acid Hybridization
nucleotide sequence
Open Reading Frames
RNA Viruses - classification
RNA Viruses - genetics
RNA, Double-Stranded - chemistry
RNA, Double-Stranded - genetics
RNA, Viral - chemistry
RNA, Viral - genetics
RNA-dependent RNA polymerase
Sequence Alignment
Virology
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Summary:A mycovirus (named FusoV) from the phytopathogenic fungus, Fusarium solani f. sp. robiniae SUF704, has two kinds of double-stranded (ds) RNA genomes, designated Ml and M2. The cDNAs were constructed from FusoV genomic dsRNAs. The sequences of Ml and M2 cDNAs comprised 1645 and 1445 bp, respectively. Sequence analysis showed that each dsRNA had a single long open reading frame (ORF) on only one of the strands. Ml ORF encodes a 519-amino acid residue polypeptide with a predicted molecular mass of 60kDa. RNA-dependent RNA polymerase-conserved motifs were identified in the predicted amino acid sequence, and the polymerase synthesized dsRNA in vitro. The M2 ORF encodes a polypeptide of 413 amino acid residues with a predicted molecular mass of 44 kDa. The predicted amino acid sequence contained the sequence corresponding to those found in the purified 44-kDa capsid protein of FusoV.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60.784