Analysis of structural components and molecular construction of soybean soluble polysaccharides by stepwise enzymatic degradation

Soybean soluble polysaccharides (SSPS) extracted from soybean cotyledons have a pectin-like structure. The core polysaccharides after treatments with four kinds of hemicellulases and a pectinase contained approximately equal numbers of L-rhamnose and D-galacturonate residues, suggesting the presence...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2001-10, Vol.65 (10), p.2249-2258
Main Authors: Nakamura, A. (Fuji Oil Co. Ltd., Izumisano, Osaka (Japan)), Furuta, H, Maeda, H, Nagamatsu, Y, Yoshimoto, A
Format: Article
Language:English
Subjects:
ARABINOSE
Arabinose - analysis
Biological and medical sciences
Carbohydrate Conformation
Carbohydrate Sequence
Chemical constitution
CHEMICAL STRUCTURE
Chromatography, Gel
Chromatography, Ion Exchange
ENZYMOLYSIS
Fundamental and applied biological sciences. Psychology
Galactose - analysis
Glycine max - chemistry
Glycoside Hydrolases - chemistry
HEMICELLULOSE
Molecular Sequence Data
pectin
PECTINS
Pectins - analysis
Plant physiology and development
POLYGALACTURONASE
Polygalacturonase - chemistry
POLYSACCHARIDES
Polysaccharides - chemistry
Polysaccharides - isolation & purification
RESIDUES
RHAMNOSE
Rhamnose - analysis
soybean
SOYBEANS
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Summary:Soybean soluble polysaccharides (SSPS) extracted from soybean cotyledons have a pectin-like structure. The core polysaccharides after treatments with four kinds of hemicellulases and a pectinase contained approximately equal numbers of L-rhamnose and D-galacturonate residues, suggesting the presence of the rhamnogalacturonan (RG) I structure consisting of the diglycosyl repeating unit, -4)-alpha-D-GalpA-(1-->2)-alpha-L-Rhap-(1-. The lengths of RG chains were calculated as approximately 15, 28, and 100 diglycosyl repeats. The RG components linked to each other by intervention of galacturonan (GN) chains, constituting the backbone of SSPS. All arabinose residues, which constitute 21% of total SSPS sugars, were found to be in side chains from RG regions, and this was also true for galactose residues, which constitute 50% of total sugars. Of arabinose residues, 94% are present as alpha-1, 3- or alpha-1, 5-arabinans, and 89% of galactose residues were present as beta-l, 4-galactans. Galactan chains are modified with arabinose, xylose, fucose, and glucose at the sites close to the RG regions.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.65.2249