Localization, accumulation, and antifungal activity of chitinases in Rye (Secale cereale) seed

In order to understand a physiological role of chitinases in rye, the localization and accumulation of rye seed chitinase-a and -c (RSC-a and -c) in the seeds were studied by immunochemical methods. An antiserum specific to the chitin-binding domain (CB-domain), which is an N-terminal part of RSC-a,...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2001-12, Vol.65 (12), p.2710-2718
Main Authors: Taira, T. (Ryukyu Univ., Nishihara, Okinawa (Japan). Coll. of Agriculture), Yamagami, T, Aso, Y, Ishiguro, M, Ishihara, M
Format: Article
Language:English
Subjects:
ALEURONE
antifungal activity
Antifungal Agents - metabolism
ANTIFUNGAL PROPERTIES
Biological and medical sciences
Biotechnology
Blotting, Western
chitin-binding domain
CHITINASE
Chitinases - metabolism
ENDOSPERM
Fundamental and applied biological sciences. Psychology
Immune Sera
Immunohistochemistry
IMMUNOLOGICAL TECHNIQUES
rye seed
Secale - embryology
Secale - enzymology
SECALE CEREALE
SEED
Seeds - metabolism
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Summary:In order to understand a physiological role of chitinases in rye, the localization and accumulation of rye seed chitinase-a and -c (RSC-a and -c) in the seeds were studied by immunochemical methods. An antiserum specific to the chitin-binding domain (CB-domain), which is an N-terminal part of RSC-a, and an antiserum specific to the catalytic region of RSC-a and RSC-c were used. An immunoblot analysis detected both RSC-a and RSC-c in the endosperm of the rye seed. Immuno-histochemical staining indicated that RSC-a was localized in only the aleurone cells, whereas RSC-c existed at least in the starchy endosperm and was also likely to exist in the aleurone cells. It was found by ELISA and an immunoblot analysis that RSC-a and -c accumulated in the seed during the later stage of development. Both chitinases and the Cat-domain exhibited antifungal activity toward Trichoderma species, while the CB-domain did not. Observation of the inhibition of hyphal growth of the T. species suggests that the two chitinases acted in different ways.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.65.2710