Isolation and characterization of alginic acid from commercially cultured Nemacystus decipiens (itomozuku) [Phaeophyceae]

An alginate was isolated from commercially cultured Nemacystus decipiens which had been harvested in Yonashiro Town (Okinawa, Japan). The yield of the alginate was 1.6% (w/w of wet alga), and the uronic acid, ash and moisture contents of the alginate were 86.0%, 12.0%, and 2.3% (w/w), respectively....

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2001, Vol.65 (3), p.654-657
Main Authors: Tako, M. (Ryukyu Univ., Nishihara, Okinawa (Japan). Coll. of Agriculture), Kiyuna, S, Uechi, S, Hongo, F
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
ALGINIC ACID
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Industrial applications and implications. Economical aspects
Itomozuku
Nemacystus decipiens
Other applications
PHAEOPHYCEAE
Plant aquaculture, algal production (macroalgae)
PLANT EXTRACTS
PURIFICATION
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Summary:An alginate was isolated from commercially cultured Nemacystus decipiens which had been harvested in Yonashiro Town (Okinawa, Japan). The yield of the alginate was 1.6% (w/w of wet alga), and the uronic acid, ash and moisture contents of the alginate were 86.0%, 12.0%, and 2.3% (w/w), respectively. The molecular mass of the alginate was estimated to be about 1.5×10 5 . The infrared spectrum and optical rotation of the alginate were in agreement with those of the standard alginate. D-Mannuronic acid and L-guluronic acid were identified by 1 H- and 13 C-NMR spectroscopy, the molar ratio of both sugar residues being estimated to be 0.72:1.00.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.65.654