Chromosomal circularization in Streptomyces griseus by nonhomologous recombination of deletion ends

Streptomyces linear chromosomes frequently cause deletions at both ends spontaneously or by various mutagenic treatments, and concomitantly display dynamic structural changes such as circularization and arm replacement. We have cloned and sequenced the fusion junctions of circularized chromosomes in...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-05, Vol.67 (5), p.1101-1108
Main Authors: Inoue, S. (Hiroshima Univ. (Japan)), Higashiyama, K, Uchida, T, Hiratsu, K, Kinashi, H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Blotting, Southern
chromosomal circularization
chromosomal deletion
CHROMOSOME ABERRATIONS
Chromosome Deletion
Chromosomes, Fungal - genetics
Culture Media
DNA HYBRIDIZATION
DNA, Fungal - biosynthesis
ELECTROPHORESIS
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
genome fluidity
Hybridization, Genetic
Molecular Sequence Data
MUTANTS
Mutation - genetics
nonhomologous recombination
NUCLEOTIDE SEQUENCE
Plasmids - genetics
RECOMBINATION
Recombination, Genetic
Spectrophotometry, Ultraviolet
Streptomyces
STREPTOMYCES GRISEUS
Streptomyces griseus - genetics
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Description
Summary:Streptomyces linear chromosomes frequently cause deletions at both ends spontaneously or by various mutagenic treatments, and concomitantly display dynamic structural changes such as circularization and arm replacement. We have cloned and sequenced the fusion junctions of circularized chromosomes in two deletion mutants of Streptomyces griseus. No homology and a 1-bp overlap were found between the deletion ends of the mutant chromosomes. Taking this together with previous results, we concluded that chromosomal circularization in Streptomyces occurs by nonhomologous recombination between deletion ends.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.67.1101