Effect of dietary pectin on the production of immunoglobulins and cytokines by mesenteric lymph node lymphocytes in mouse colitis induced with dextran sulfate sodium

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-08, Vol.67 (8), p.1706-1712
• Main Authors: Lim, B.O. (Kyung Hee Univ., Seoul (Korea R.)), Lee, S.H, Park, D.K, Choue, R.W
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Body Weight; COLITIS; Colitis - chemically induced; Colitis - immunology; CYTOKINES; Cytokines - biosynthesis; Cytokines - metabolism; dextran sodium sulfate; Dextran Sulfate; DEXTRANS; DIET; Dietary Fiber - pharmacology; dietary pectin; Eating; FAECES; fecal IgA; Feces - chemistry; Female; Fundamental and applied biological sciences. Psychology; Immunoglobulin A - biosynthesis; Immunoglobulin E - biosynthesis; IMMUNOGLOBULINS; inflammatory bowel disease; Lymph Nodes - cytology; Lymph Nodes - drug effects; Lymph Nodes - immunology; LYMPHATIC SYSTEM; Lymphocytes - drug effects; Lymphocytes - immunology; mesenteric lymph node; Mesentery - drug effects; Mesentery - immunology; MICE; Mice, Inbred BALB C; PECTINS; Pectins - pharmacology; PERITONEUM; Time Factors
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.67.1706

The present study explores the dietary effect of pectin on the MLN lymphocyte functions of mice with dextran sulfate sodium (DS)-induced colitis. We found that the immunoglobulin (Ig)A level in mesenteric lymph node (MLN) lymphocytes was high, while the IgE level was lower, in mice fed with pectin than in those fed with cellulose. Interestingly, the fecal IgA concentration of the pectin-fed mice was significantly higher than that of the cellulose-fed mice. The concentrations of interferong and interleukin (IL)-2 treated with concanavalin A (ConA) were significantly higher in the pectin-fed group than in the cellulose-fed group. Although dietary pectin did not affect the IL-4 and IL-10 levels, the activationinduced IL-4 and IL-10 secretion was lower in MLN cells of the pectin-fed mice than of the cellulose-fed mice following DS-induced colitis. Based on these findings, we propose that the effect of dietary pectin on mice with DS-induced colitis is mediated by the manipulation of Th1 cells. Furthermore, the inhibitory effect of IL-4 and IL-10 by dietary pectin may play an important role in promoting a change in Th1/Th2 balance toward Th1dominant immunity.