Malformation of immature starfish oocytes by theonellapeptolide Ie, a tridecapeptide lactone from a marine sponge Petrosia species, through disturbance of cortical F-actin distribution

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-09, Vol.67 (9), p.1908-1915
• Main Authors: Ohta, E. (Hiroshima Univ. (Japan)), Okada, H, Ohta, S, Kobayashi, M, Kitagawa, I, Horiike, S, Takahashi, T, Hosoya, H, Yamamoto, K, Ikegami, S
• Format: Article
• Language: English
• Subjects: ACTIN; Actins - metabolism; Actins - ultrastructure; Adenine - analogs & derivatives; Adenine - pharmacology; ANIMAL MORPHOLOGY; Animals; Asterina pectinifera; ASTEROIDEA; Biological and medical sciences; Cytochalasin D - pharmacology; Dose-Response Relationship, Drug; Enzyme Activation - drug effects; Fundamental and applied biological sciences. Psychology; Growth Substances - pharmacology; LACTONES; MALFORMATIONS; Marine; MATURITY; MEIOSIS; Microscopy - methods; oocyte; Oocytes - cytology; Oocytes - drug effects; Oocytes - growth & development; Oocytes - ultrastructure; OVA; PARAZOA; PEPTIDES; Peptides - chemistry; Peptides - toxicity; Petrosia; Porifera - chemistry; Protein Kinases - metabolism; starfish; Starfish - physiology; theonellapeptolide Ie; Time Factors
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.67.1908

Theonellapeptolide Ie (Tp), an oligopeptide lactone isolated from a marine sponge, Petrosia sp., was shown to induce an unprecedented morphological change in the immature oocytes of the starfish Asterina pectinifera. The cortical F-actin was disturbed and assembled to form dots and rings, as evidenced by staining with rhodamine-conjugated phalloidin. The oocyte eventually became malformed. When Tp was added to an immature oocyte which had been pretreated with cytochalasin B or D, inhibitors of actin polymerization, no malformation was observed. When Tp was added to an oocyte which had been induced to mature by 1-methyladenine (1-MeAde), a maturation-induciDg substance in starfishes, no morphological changes were observed in the maturing oocytes which reached the first meiotic prometaphase 40 min after the start of 1-MeAde treatment. This is the first description of a chemical that induces aberrant redistribution of F-actin-based cytoskeleton in an animal oocyte which is arrested at the first meiotic prophase.