Corn silk induced cyclooxygenase-2 in murine macrophages

Stimulation of murine macrophages with corn silk induced cyclooxygenase (COX)-2 with secretion of PGEsub(2). Expression of COX-2 was inhibited by pyrolidine dithiocarbamate (PDTC), and increased DNA binding by nuclear factor kappa B (NF-kappaB), indicating that COX-2 induction proceeds also via the...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-10, Vol.69 (10), p.1848-1853
Main Authors: Kim, K.A.(University of Ulsan (Korea R.)), Shin, H.H, Choi, S.K, Choi, H.S
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
corn silk
cyclooxygenase (COX-2)
Cyclooxygenase 2 - genetics
Dinoprostone - physiology
Dinoprostone - secretion
ENZIMAS
ENZYME
ENZYMES
Feedback, Physiological
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Enzymologic
IMMUNOBLOTTING
inducible nitric oxide synthase (iNOS)
LIGASAS
LIGASE
LIGASES
MACROFAGOS
MACROPHAGE
MACROPHAGES
MICE
NF-kappa B - metabolism
NITRIC OXIDE
Nitric Oxide Synthase Type II - genetics
OXIDO NITRICO
OXYDE NITRIQUE
PGE
Plant Structures
Plants, Medicinal
PROSTAGLANDINAS
PROSTAGLANDINE
PROSTAGLANDINS
PURIFICACION
PURIFICATION
RATON
Receptors, Prostaglandin E - physiology
Receptors, Prostaglandin E, EP2 Subtype
Receptors, Prostaglandin E, EP4 Subtype
SEDA
SILK
SOIE
SOURIS
ZEA MAYS
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Summary:Stimulation of murine macrophages with corn silk induced cyclooxygenase (COX)-2 with secretion of PGEsub(2). Expression of COX-2 was inhibited by pyrolidine dithiocarbamate (PDTC), and increased DNA binding by nuclear factor kappa B (NF-kappaB), indicating that COX-2 induction proceeds also via the NF-kappaB signaling pathway. A specific inhibitor of COX-2 decreased the expression level of inducible nitric oxide synthase (iNOS) stimulated by corn silk. PGEsub(2) elevated the expression level of iNOS, probably via EP2 and EP4 receptors on the surface of the macrophages.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.69.1848