Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S. coelicolor A3(2)

Streptomyces coelicolor A3(2) has 13 chitinase genes encoding 11 family 18 and two family 19 chitinases. To compare enzymatic properties of family 19 chitinase and family 18 chitinases produced by the same organism, the four chitinases (Chi18bA, Chi18aC, Chi18aD, and Chi19F), whose genes are express...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-04, Vol.70 (4), p.988-998
Main Authors: Kawase, T.(Niigata Univ. (Japan). Faculty of Agriculture), Yokokawa, S, Saito, A, Fujii, T, Nikaidou, N, Miyashita, K, Watanabe, T
Format: Article
Language:English
Subjects:
ANALISIS ENZIMATICO
ANALYSE ENZYMATIQUE
antifungal activity
Antifungal Agents - classification
Antifungal Agents - isolation & purification
Antifungal Agents - metabolism
Antifungal Agents - pharmacology
ANTIFUNGAL PROPERTIES
Biological and medical sciences
CHITINASE
Chitinases - classification
Chitinases - isolation & purification
Chitinases - metabolism
Chitinases - pharmacology
ENZYMATIC ANALYSIS
Enzyme Stability
family 19
Fundamental and applied biological sciences. Psychology
GENE
GENES
Hydrogen-Ion Concentration
Hydrolysis
PROPIEDADES ANTIMICOSICAS
PROPRIETE ANTIFONGIQUE
QUITINASA
STREPTOMYCES
Streptomyces coelicolor
Streptomyces coelicolor - enzymology
Streptomyces coelicolor - genetics
Streptomyces coelicolor A3
Substrate Specificity
Temperature
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Summary:Streptomyces coelicolor A3(2) has 13 chitinase genes encoding 11 family 18 and two family 19 chitinases. To compare enzymatic properties of family 19 chitinase and family 18 chitinases produced by the same organism, the four chitinases (Chi18bA, Chi18aC, Chi18aD, and Chi19F), whose genes are expressed at high levels in the presence of chitin, were produced in Eschericha coli and purified. The effect of pH on the hydrolytic activity was very different not only among the four chitinases but also among the substrates. The hydrolytic activity of Chi19F, family 19 chitinase, against soluble substrates was remarkably high as compared with three family 18 chitinases, but was the lowest against crystalline substrates among the four chitinases. On the contrary, Chi18aC, a family 18-subfamily A chitinase, showed highest activity against crystalline substrates. Only Chi19F exhibited significant antifungal activity. Based on these observations, the roles of family 19 chitinases are discussed.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.70.988