RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment

High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2008-07, Vol.72 (7), p.1951-1953
Main Authors: Suzuki, Y.(Tohoku Univ., Sendai (Japan)), Mae, T, Makino, A
Format: Article
Language:English
Subjects:
ARN
Biological and medical sciences
Bis-Trimethylammonium Compounds
Buffers
Chloroform
ELECTROFORESIS
ELECTROPHORESE
ELECTROPHORESIS
EXTRACCION
EXTRACTION
FEUILLE
FLEUR
FLORES
FLOWERS
Fundamental and applied biological sciences. Psychology
Guanidines
HOJAS
LEAVES
Methods
ORGANE DE RESERVE
ORGANOS DE RESERVA
PCR
Phenol
Plant Structures - chemistry
PLANT TISSUES
POLISACARIDOS
POLYHOLOSIDE
POLYSACCHARIDES
RNA
RNA extraction
RNA, Plant - isolation & purification
STORAGE ORGANS
storage tissues
TEJIDOS VEGETALES
Thiocyanates
TISSU VEGETAL
tree leaves
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Summary:High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds of Lotus japonicus and rice, sweet potato tuber, banana fruit). This protocol greatly reduced the time required for RNA extraction.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.80084