RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment

High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2008-07, Vol.72 (7), p.1951-1953
Main Authors: Suzuki, Y.(Tohoku Univ., Sendai (Japan)), Mae, T, Makino, A
Format: Article
Language:English
Subjects:
ARN
Biological and medical sciences
Bis-Trimethylammonium Compounds
Buffers
Chloroform
ELECTROFORESIS
ELECTROPHORESE
ELECTROPHORESIS
EXTRACCION
EXTRACTION
FEUILLE
FLEUR
FLORES
FLOWERS
Fundamental and applied biological sciences. Psychology
Guanidines
HOJAS
LEAVES
Methods
ORGANE DE RESERVE
ORGANOS DE RESERVA
PCR
Phenol
Plant Structures - chemistry
PLANT TISSUES
POLISACARIDOS
POLYHOLOSIDE
POLYSACCHARIDES
RNA
RNA extraction
RNA, Plant - isolation & purification
STORAGE ORGANS
storage tissues
TEJIDOS VEGETALES
Thiocyanates
TISSU VEGETAL
tree leaves
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cited_by cdi_FETCH-LOGICAL-c474t-bde6686e8f0227552127dbbfc25b2eac4eec2b4ace98c9f522a084654b89f4843
cites cdi_FETCH-LOGICAL-c474t-bde6686e8f0227552127dbbfc25b2eac4eec2b4ace98c9f522a084654b89f4843
container_end_page 1953
container_issue 7
container_start_page 1951
container_title Bioscience, biotechnology, and biochemistry
container_volume 72
creator Suzuki, Y.(Tohoku Univ., Sendai (Japan))
Mae, T
Makino, A
description High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds of Lotus japonicus and rice, sweet potato tuber, banana fruit). This protocol greatly reduced the time required for RNA extraction.
doi_str_mv 10.1271/bbb.80084
format article
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identifier ISSN: 0916-8451
ispartof Bioscience, biotechnology, and biochemistry, 2008-07, Vol.72 (7), p.1951-1953
issn 0916-8451
1347-6947
language eng
recordid cdi_crossref_primary_10_1271_bbb_80084
source Oxford Journals Online; EZB Electronic Journals Library
subjects ARN
Biological and medical sciences
Bis-Trimethylammonium Compounds
Buffers
Chloroform
ELECTROFORESIS
ELECTROPHORESE
ELECTROPHORESIS
EXTRACCION
EXTRACTION
FEUILLE
FLEUR
FLORES
FLOWERS
Fundamental and applied biological sciences. Psychology
Guanidines
HOJAS
LEAVES
Methods
ORGANE DE RESERVE
ORGANOS DE RESERVA
PCR
Phenol
Plant Structures - chemistry
PLANT TISSUES
POLISACARIDOS
POLYHOLOSIDE
POLYSACCHARIDES
RNA
RNA extraction
RNA, Plant - isolation & purification
STORAGE ORGANS
storage tissues
TEJIDOS VEGETALES
Thiocyanates
TISSU VEGETAL
tree leaves
title RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment
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