Fluorescence imaging of cells using long-range electromagnetic surface waves for excitation

We present a depth-localized illumination technique for wide-field fluorescence microscopy, based on long-range optical surface waves. This technique allows one to excite the fluorescence only in a thin near-substrate layer of the specimen. Our experimental setup is compatible with both upright and...

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Bibliographic Details
Published in:Applied optics (2004) 2020-06, Vol.59 (16), p.4833
Main Authors: Prusakov, K A, Bagrov, D V, Basmanov, D V, Romanov, S A, Klinov, D V
Format: Article
Language:English
Subjects:
Electromagnetic Radiation
Equipment Design
Escherichia coli
Green Fluorescent Proteins - metabolism
HeLa Cells
Humans
Lasers
Light
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Optical Imaging
Optics and Photonics
Signal-To-Noise Ratio
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Summary:We present a depth-localized illumination technique for wide-field fluorescence microscopy, based on long-range optical surface waves. This technique allows one to excite the fluorescence only in a thin near-substrate layer of the specimen. Our experimental setup is compatible with both upright and inverted microscopes. It provides fluorescent microscopic images, which are superior to the epifluorescence ones in signal-to-noise ratio, contrast, and detail. We demonstrate the applicability of our technique for imaging both bacterial and eukaryotic cells (E. coli and HeLa, respectively).
ISSN:1559-128X
2155-3165
DOI:10.1364/AO.389120