Broadband stimulated Raman hyperspectral imaging of cells and semiconductors with synchronized fiber and solid-state sources

We demonstrate broadband hyperspectral stimulated Raman scattering (SRS) microscopy covering over 2000 cm −1 , which is achieved by two tunable Stokes light sources synchronized with a pump light source. Specifically, a fiber optical parametric oscillator and a fiber laser, both equipped with automa...

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Bibliographic Details
Published in:Optics express 2025-02, Vol.33 (3), p.3890
Main Authors: Takahashi, Shun, Kamei, Kento, Oguchi, Kenichi, Kuruma, Kazuhiro, Spratt, Spencer J., Akaboshi, Hikaru, Wakamoto, Yusuke, Maeda, Takuya, Ozeki, Yasuyuki
Format: Article
Language:English
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Summary:We demonstrate broadband hyperspectral stimulated Raman scattering (SRS) microscopy covering over 2000 cm −1 , which is achieved by two tunable Stokes light sources synchronized with a pump light source. Specifically, a fiber optical parametric oscillator and a fiber laser, both equipped with automatic wavelength tuning capabilities, are employed to acquire SRS signals in the ranges of 270–2040 cm −1 and 2800–3100 cm −1 , respectively. Using this system, we perform hyperspectral SRS imaging of live HeLa cells, covering the entire fingerprint and C–H stretching regions. Furthermore, the spectral coverage in the lower fingerprint region (
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.545877