Determination of Palmatine in Rabbit Plasma by RP-HPLC with Solid-Phase Extraction

A rapid and specific reversed-phase high performance liquid chromatography (RP-HPLC) method for the determination of palmatine in rabbit plasma has been developed and validated. The chromatographic separation was performed on a C₁₈ column at 40 °C. The mobile phase, delivered at 1.0 mL min⁻¹, consis...

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Bibliographic Details
Published in:Chromatographia 2005-11, Vol.62 (9-10), p.471-474
Main Authors: Huang, Jianming, Weng, Weiyu, Cao, Di, Huang, Xubing, Wang, Jian, Yu, Hao
Format: Article
Language:English
Subjects:
acetonitrile
Analysis
berberine
Biological and medical sciences
detection limit
General pharmacology
intravenous injection
Medical sciences
Pharmacology. Drug treatments
phosphates
rabbits
reversed-phase high performance liquid chromatography
solid phase extraction
wavelengths
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Summary:A rapid and specific reversed-phase high performance liquid chromatography (RP-HPLC) method for the determination of palmatine in rabbit plasma has been developed and validated. The chromatographic separation was performed on a C₁₈ column at 40 °C. The mobile phase, delivered at 1.0 mL min⁻¹, consisted of acetonitrile/phosphate buffer (pH 3.0) 40:60 (v/v). The detection wavelength was set at 345 nm. Palmatine and internal standard (IS) berberine were extracted from plasma by solid-phase extraction using C18 cartridges. Linearity was confirmed in the concentration range of 0.01 to 5 μg mL⁻¹, the inter-day and intra-day RSDs were within 10.0, the recoveries of palmatine ranged from 93.1 to 110.3, and the limit of detection (LOD, S/N > 3) was 0.002 μg mL⁻¹. The method is applicable to the determination of palmatine in rabbit plasma after intravenous administration of palmatine.
ISSN:0009-5893
1612-1112
DOI:10.1365/s10337-005-0663-z