HPLC Method for Determination of 3,4-Diaminopyridine in the Presence of Related Substances and Degradation Products Formed Under Stress Conditions

3,4-Diaminopyridine is used to treat some symptoms met in Lambert Eaton myasthenia syndrome. It was shown efficient to reduce a form of variable muscle weakness and fatigability typical of the disease and correlated to a block of acetylcholine release. A high performance liquid chromatographic metho...

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Bibliographic Details
Published in:Chromatographia 2006-06, Vol.63 (11-12), p.599-603
Main Authors: Do, B, Goulay-Dufaÿ, S, Hoang, M. D. Le, Raust, J. A, Guyon, F, Graffard, H, Pradeau, D
Format: Article
Language:English
Subjects:
acetonitrile
acetylcholine
ammonium acetate
Analysis
aqueous solutions
Biological and medical sciences
General pharmacology
high performance liquid chromatography
Medical sciences
muscles
Pharmacology. Drug treatments
sodium
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Summary:3,4-Diaminopyridine is used to treat some symptoms met in Lambert Eaton myasthenia syndrome. It was shown efficient to reduce a form of variable muscle weakness and fatigability typical of the disease and correlated to a block of acetylcholine release. A high performance liquid chromatographic method for the determination of 3,4-diaminopyridine in the presence of related substances and its degradation products is described. The method is based on the use of a C18 bonded phase column and a mobile phase composed of 10 volumes of acetonitrile and 90 volumes of an aqueous solution containing 1 g L⁻¹ sodium octanesulfonate and 0.77 g L⁻¹ ammonium acetate. The pH of the aqueous solution was adjusted to 1.9 with trifluoracetic acid. All peaks are eluted in
ISSN:0009-5893
1612-1112
DOI:10.1365/s10337-006-0821-y