Validated HPLC Method with Fluorescence Detection for the Determination of Droperidol in Pharmaceutical Tablets, Human Serum, and Human Milk

• Published in: Chromatographia 2007-09, Vol.66 (S1), p.37-43
• Main Authors: Yeniceli, D, Dogrukol-Ak, D, Tuncel, M
• Format: Article
• Language: English
• Subjects: droperidol; Fluorimetric detection; liquid chromatography; Pharmaceuticals in human serum and human milk
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-007-0255-1

A sensitive and simple HPLC method with fluorimetric detection has been developed for determination of droperidol in pharmaceutical tablets, human serum, and human milk. Chromatography was performed on a 100 mm x 3 mm i.d. C₁₈ column with methanol-water, 30:70 (v/v), pH 3.5, as mobile phase at a flow-rate of 0.8 mL min-¹. The injection volume was 5 μL and detection was by monitoring emission at 324 nm after excitation at 283 nm. Droperidol and p-hydroxybenzoic acid (internal standard) eluted after 5.3 and 6.1 min, respectively. The method was validated over the concentration range 1.14 x 10-⁷ to 9.12 x 10-⁶ M. Selectivity was good and the limits of detection and quantitation of the method were approximately 3.54 x 10-⁸ and 1.07 x 10-⁷ M, respectively, corresponding to 13 and 40 ng mL-¹. The applicability of the method to determination of droperidol in pharmaceuticals, human serum, and human milk was demonstrated.