Determination of Abamectin Residues in Avocados by Microwave-Assisted Extraction and HPLC with Fluorescence Detection

In this article a new analytical method for the confirmation and quantification of abamectin residues in avocados is described. The method allows a fast analysis of abamectin homologues using microwave assisted extraction (MAE), solid-phase extraction (SPE) and high-performance liquid chromatography...

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Bibliographic Details
Published in:Chromatographia 2008, Vol.67 (1-2), p.69-75
Main Authors: Hernández Borges, Javier, Ravelo-Pérez, Lidia M, Hernández-Suárez, Estrella M, Carnero, Aurelio, Rodríguez-Delgado, Miguel Ángel
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biological and medical sciences
Chemistry
Chemistry and Materials Science
Chromatography
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Laboratory Medicine
Original
Pharmacy
Proteomics
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Summary:In this article a new analytical method for the confirmation and quantification of abamectin residues in avocados is described. The method allows a fast analysis of abamectin homologues using microwave assisted extraction (MAE), solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) with fluorescence (FL) detection using trifluoroacetic anhydride (TFAA) and N-methylimidazole (NMIM) as derivatizing agents. The mobile phase consisted of water, methanol and acetonitrile (5:47.5:47.5 v/v/v) and was pumped at a rate of 1.1 mL min-¹ (isocratic elution). Homogenized avocado samples were extracted once with 20 mL acetonitrile:water 4:1 (v/v) in a microwave oven for 26 min at 700 W with a maximum temperature of 80 °C. MAE operational parameters were optimized by means of an experimental design. Extracts were cleaned using C₁₈ SPE cartridges. Average recoveries of the method at four spiked levels (0.005, 0.01, 0.10 and 1.0 mg kg-¹) were found to be in the range 90-100% with good precision (RSD < 12%). The limits of detection (LODs) and quantification (LOQs) of the whole method were 0.001 and 0.003 mg kg-¹, respectively, which are lower than the maximum residue limit (MRL) established by the Spanish and the European legislation in avocados (0.01 mg kg-¹). Several avocado samples previously treated with the pesticide were also analyzed.
ISSN:0009-5893
1612-1112
DOI:10.1365/s10337-007-0442-0